TC總膽固醇檢測試劑盒 | Total Cholesterol (TC) Colorimetric Assay Kit貨號KTB2220
CheKine™ Total Cholesterol (TC) Colorimetric Assay Kit-快速檢測血清，血漿，組織，細胞和細菌中總膽固醇(Total Cholesterol, TC)濃度
該試劑盒提供了一種簡單的方法來檢測各種生物樣品（例如血清，血漿，組織，細胞和細菌）中的總膽固醇(Total Cholesterol, TC)濃度。在測定中，酯酶(esterase)催化膽固醇酯(cholesterol esters)的水解，生成游離膽固醇（free cholesterol , FC）和游離脂肪酸（free fatty acids , FFA）。然後膽固醇氧化酶(Cholesterol oxidase)催化游離膽固醇（free cholesterol , FC）產生△4-膽固醇(△4-cholestenone)和過氧化氫(H2O2)。另外，過氧化物酶(peroxidase)可以催化H 2 O 2，4-氨基安替比林(4-aminoantipyrine)和苯酚(phenol)，形成在500nm處具有特徵吸收峰的紅色醌化合物(red quinone compounds)。樣品中的總膽固醇(Total Cholesterol, TC)與獲得的信號成比例。總膽固醇（Total Cholesterol, TC）包括游離膽固醇( free cholesterol )和膽固醇酯(cholesterol esters)。組織總膽固醇（Tissue total cholesterol, TC）是指組織中所有脂蛋白( lipoproteins )所含膽固醇( cholesterol )的總和。請使用500nm ELISA reader檢測; 若需使用分光光度計, 需更換為微量cuvette。
The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA). Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.Total cholesterol (TC) includes free cholesterol and cholesterol esters. Tissue total cholesterol (TC) refers to the sum of cholesterol contained in all lipoproteins in the tissue.
| Product name | CheKine™ Micro Total Cholesterol (TC) Assay Kit |
| Applications notes | The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA). Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained. |
| Alternative | TC; cholesterol |
| Kit components | • TC Working solution • Standard |
| Features & Benefits | • Determination of Total Cholesterol (TC) content in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. • Detailed sample preparation and result calculation methods are provided. • Simple operation steps. |
| Usage notes | • Do not mix or substitute reagents or materials from other kit lots or vendors. • There are volatile substances in the kit, gloves and masks should be worn during the experiment, and the reagent bottle cap should be tightly closed in time after opening. • Ensure all reagents and equipment are at the appropriate temperature before starting the assay. |
| Storage instructions | Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Total cholesterol (TC) includes free cholesterol and cholesterol esters. Tissue total cholesterol (TC) refers to the sum of cholesterol contained in all lipoproteins in the tissue. |
| Alternative | TC; cholesterol |
客戶自備材料: Materials Required but Not Supplied ·Microplate reader or visible spectrophotometer capable of measuring absorbance at 500 nm ·96-well plate or microglass cuvette, precision pipettes, disposable pipette tips ·Ice maker, refrigerated centrifuge, water bath ·Anhydrous ethanol ·Homogenizer (for tissue samples)。
KTB2220試劑盒可以用於檢測雞蛋樣本，蛋白樣本可直接測定; 蛋黃樣本按操作手冊中，組織樣本處理方法製備即可。(如下所示) 樣品製備注意：建議使用新鮮樣品，如不立即檢測，樣品可在-80℃保存一個月。(一)動物組織：稱取約0.1g組織，加入1mL無水乙醇。在冰上均質化。 4℃ 8,000g 離心 10 分鐘。使用上清液進行測定，並將其置於冰上進行測試。(二) 細胞或細菌：收集 5×106.將細胞或細菌放入離心管中，離心後棄上清；加入1mL無水乙醇在冰浴中超聲破碎細胞或細菌5分鐘（功率20%或200W，超聲3s，間隔7s，重複30次）。 4℃ 8,000 g 離心 10 分鐘。使用上清液進行測定，並將其置於冰上進行測試。(三) 血清（血漿）樣品：直接檢測。注：如果以蛋白濃度計算含量，最好用Protein Quantification Kit (BCA Assay), Cat #: KTD3001對總蛋白進行定量。
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