TC總膽固醇檢測試劑盒 | 貨號KTB2220

CheKine™ Total Cholesterol (TC) Colorimetric Assay Kit

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該試劑盒提供了一種簡單的方法來檢測各種生物樣品(例如血清,血漿,組織,細胞和細菌)中的總膽固醇(Total Cholesterol, TC)濃度。在測定中,酯酶催化膽固醇酯(cholesterol esters)的水解,生成游離膽固醇(free cholesterol , FC)和游離脂肪酸(free fatty acids , FFA)。然後膽固醇氧化酶(Cholesterol oxidase)催化游離膽固醇(free cholesterol , FC)產生△4-膽固醇(△4-cholestenone)和過氧化氫(H2O2)。另外,過氧化物酶(peroxidase)可以催化H 2 O 2,4-氨基安替比林(4-aminoantipyrine)和苯酚(phenol),形成在500nm處具有特徵吸收峰的紅色醌化合物(red quinone compounds)。樣品中的總膽固醇(Total Cholesterol, TC)與獲得的信號成比例。

Product name CheKine™ Total Cholesterol (TC) Colorimetric Assay Kit
Applications notes The kit provides a simple method for detecting TC concentration in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria. In the assay, esterase catalyzes the hydrolysis of cholesterol esters to produce free cholesterol (FC) and free fatty acids (FFA). Then Cholesterol oxidase catalyzes free cholesterol (FC) to produce △4-cholestenone and H2O2. Further, H2O2, 4-aminoantipyrine and phenol can be catalyzed by peroxidase to form red quinone compounds which has a characteristic absorption peak at 500 nm. The Total Cholesterol (TC) present in the sample is proportional to the signal obtained.
Alternative TC; cholesterol
  • Kit components
  • TC Working solution
    • Standard
  • Features & Benefits
  • Determination of Total Cholesterol (TC) content in a variety of biological samples such as serum, plasma, tissues, cells, and bacteria.
    • Detailed sample preparation and result calculation methods are provided.
    • Simple operation steps.
  • Usage notes
  • Do not mix or substitute reagents or materials from other kit lots or vendors.
    • There are volatile substances in the kit, gloves and masks should be worn during the experiment, and the reagent bottle cap should be tightly closed in time after opening.
    • Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
  • Storage instructions

Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.

  • Shipping

Gel pack with blue ice.

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貨號 產品名稱 胞器染色位置 螢光波段  
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貨號KTC4100 Pro活細胞微管蛋白染色 微管蛋白(Tubulin) Ex/Em = 500/520 nm 活細胞或固定之細胞
貨號KTC4001 Abbkine 細胞膜染色  細胞膜 (plasma membrane) Ex/Em = 484/501 nm 活細胞、固定懸浮、貼附細胞
貨號BMD00063 DAPI Staining Solution 細胞核 dsDNA Ex/Em: 358/461 nm (with DNA)  染色活細胞和固定細胞
貨號KTC4210 活細胞Lysosome染色 Lysosome deep red fluorescent dye , Ex/Em:650/665nm  染色活細胞和固定細胞

 

 

 

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