25-OH-D(25羥基維生素D)ELISA試劑盒 -ELK Biotechnology貨號ELK3725
25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Competitive Inhibition，偵測的靈敏度3.13-200 ng/mL、最低靈敏度0.91 ng/mL
產品商品:
25-OH-D(25羥基維生素D)ELISA試劑盒 | 貨號ELK3725 (Competitive Inhibition)，偵測的靈敏度3.13-200 ng/mL、最低靈敏度0.91 ng/mL。ELISA原理為利用抗體與抗原之間的高度專一性，做為定量標誌的方法；無論測量抗原或測量抗體都有很高的靈敏度跟專一性。該檢測方法對小鼠25-OH-D(25羥基維生素D)具有高靈敏度和出色的特異性。ELISA商品的型式多元， 提供三明治ELISA、競爭型ELISA。可應用於serum, plasma, tissue homogenates, cell lysates, cell culture。並在 450nm ± 10nm 的波長下用分光光度計測量顏色變化。然後通過將樣品的 OD 與標準曲線進行比較來確定樣品中25-OH-D(25羥基維生素D)的濃度。
| Product name: | 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit |
| Reactivity: | General |
| Alternative Names: | 25 Hydroxy Vitamin D; |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 0.91 ng/mL |
| Standard: | 200 ng/mL |
| Range: | 3.13-200 ng/mL |
| Sample Type: | serum, plasma and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism; |
| Test principle: | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25 Hydroxy Vitamin D(25-OH-D) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25 Hydroxy Vitamin D(25-OH-D). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 25 Hydroxy Vitamin D(25-OH-D) in the samples is then determined by comparing the OD of the samples to the standard curve. |
標準品濃度200 ng/mL
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 200.00 | 0.199 | — |
| 100.00 | 0.357 | — |
| 50.00 | 0.587 | — |
| 25.00 | 0.823 | — |
| 12.50 | 1.253 | — |
| 6.25 | 1.596 | — |
| 3.13 | 1.871 | — |
| 0.00 | 2.233 | — |
實驗流程概念
ELK原廠網址 >> http://www.elkbiotech.com/
ELK Biotechnology公司是一家專注於生命科學、免疫研究的高科技生物企業，位於Biolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology是一家專注於免疫產品和分子生物學產品研發和生產的高科技生物公司。產品涵蓋ELISA試劑盒、抗體、基因組提取試劑、分子生物學試劑。涉及免疫化學、腫瘤研究、神經生物學、細胞週期、信號轉導等領域。我們的產品有嚴格的研發和質量檢測流程。每個產品都有完整的研發、生產和質檢記錄。經驗豐富的研發人員，完善的質檢支持部門，嚴格的檢測流程，為客戶提供質量可靠的科研產品。
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