太鼎生物科技/太鼎食安科技

組蛋白去乙醯酶3（Histone Deacetylase 3）抑制劑篩選套組

HDAC3 Inhibitor Screening Kit
Catalog#: K363-100  | Size: 100 assays

 Kit Summary: 
• Detection method- Fluorescence (Em/Ex = 380/500 nm) 
• Sample type- Testing candidate HDAC3 inhibitors 
• Applications- Suitable for high throughput screening of HDAC3 inhibitors

Features & Benefits: 
• Simple procedure; takes ~ 60 min 
• Fast and convenient 
• Kit contains the necessary reagents for testing putative HDAC3 inhibitors

Kit Components: 
• HDAC3 Assay Buffer 
• HDAC3 Substrate 
• HDAC-3 Enzyme 
• Developer 
• Trichostatin A

Description: 
Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC-3 Inhibitor screening Kit, HDAC-3 and developer will deacetylate and cleave the substrate [Arg-His-Lys-Lys(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex=380/500 nm. In presence of a HDAC-3 inhibitor, the cleavage will be inhibited. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC-3 inhibitors. Trichostatin A (TSA) is included as a control inhibitor to compare the efficacy of the test inhibitors.

Storage Conditions: 
-20°C (Upon receipt place HDAC-3 Enzyme at -80 °C for storage.)

Shipping Conditions: 
gel pack