太鼎生物科技/太鼎食安科技

G1P 呈色分析套組

Glucose-1-Phosphate (G1P) Colorimetric Assay Kit

Catalog#: K697-100| Size:100 Assays

葡萄糖-1 - 磷酸（G1P）是葡萄糖代謝和儲存的碳水化合物中，一種重要的中間體。測量細胞內含量，用於分析的碳水化合物代謝途徑和其動力學性質。葡萄糖-1 - 磷酸的測定，G1P轉換為葡萄糖-6 - 磷酸葡萄糖磷酸葡萄糖的存在下，1,6 - 二磷酸，葡萄糖-6 - 磷酸，其後由葡萄糖-6 - 磷酸脫氫酶氧化形成NADH，從而降低了有色產物具有較強的450nm處的吸光度為無色探針。 BioVision的G1P檢測試劑盒快速，靈敏和易於使用及可檢測1μm到10毫米G1P。此G1P檢測試劑盒可用於為多種類型的樣品。

產品資料

Assay
Kit Name:

Glucose-1-Phosphate (G1P) Colorimetric Assay Kit

Kit
Summary:

• Detection method- Absorbance (450 nm)

• Species reactivity- Mammalian

• Applications- The kit can detect glucose-1-phosphate concentrations in the
range of 1 µM – 10 mM .

Sample
Type:

Animal tissues: Liver, muscle and heart etc. Cell culture: Adherent or
suspension cells.

Features
& Benefits:

• Simple procedure; takes ~ 40 minutes

• Rapid, convenient and sensitive

• Kit contains all necessary reagents for accurate measurement of
glucose-1-phosphate levels

Kit
components:

• G1P Assay Buffer

• G1P Enzyme Mix (Lyophilized)

• G1P Developer (Lyophilized)

• G1P Substrate Mix (Lyophilized)

• G1P Standard (Lyophilized)

Description:


Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose
metabolism and storage. In response to hormonal or neural signals,
glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is
released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate
is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and
enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen
through the actions of Phosphoglucose isomerase (G6P -> G1P),
UDPG-pyrophosphorylase (G1P -> UDP-glucose) and glycogen synthase
(UDP-glucose + glycogen[n] -> UDP + glycogen[n+1]). Glucose-1-phosphate is
found in virtually all organisms from bacteria to higher plants and animals.
Measurement of intracellular G1P levels is crucial for analyzing the
carbohydrate metabolic pathways and their kinetic properties. In
Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase
in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently
oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a
colorless probe to a colored product with strong absorbance at 450 nm.
BioVision’s Glucose-1-phosphate assay kit is rapid, sensitive & easy to use
& can detect 1µM to 10 mM
G1P. This G1P assay kit can be used for a variety of sample types.

Storage
Conditions:

-20°C

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【連絡人】許虹宜 0920-312382 Hung-Yi Hsu

【E-mail】taiding.biotech@msa.hinet.net

【公司電話】02-86609496【公司傳真】02-86609342

【公司網址】www.biopioneer.com.tw

【原廠網址】www.biovision.com