太鼎生物科技/太鼎食安科技

HSPG2 ELISA Kit

[ INTENDED USE ] Manual

The kit is a sandwich enzyme
immunoassay for in vitro quantitative measurement of HSPG2 in human serum,

plasma,
tissue homogenates and other biological fluids.

[ TEST PRINCIPLE ]

The microtiter plate provided
in this kit has been pre-coated with an antibody specific to HSPG2. Standards
or

samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated antibody
preparation

specific for HSPG2. Next,
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate
well

and incubated. After TMB
substrate solution is added, only those wells that contain HSPG2,
biotin-conjugated

antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate reaction is

terminated by the addition of
sulphuric acid solution and the color change is measured spectrophotometrically
at

a wavelength of 450nm ± 10nm.
The concentration of HSPG2 in the samples is then determined by comparing

the O.D.
of the samples to the standard curve.

[供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 (Hung-Yi Hsu)

【E-mail】taiding.biotech@msa.hinet.net

【臺北公司】02-86609496

【相關網址】www.biopioneer.com.tw

【原廠網址】

www.uscnk.com