太鼎生物科技/太鼎食安科技

Caspase-3
Colorimetric Assay Kit

Caspase-3活性分析套組

Catalog#:
K106-100 | Size: 100 assays

Kit
Summary:

• Detection method- Absorbance (400 or 405 nm)

• Sample type- Cell and tissue lysates

• Species reactivity- Mammalian

• Kit size- Convenient sizes (25, 100, 200, 400 assays)

• Applications- Detect early/middle stages of apoptosis; differentiate
apoptosis from necrosis.

Features
& Benefits:

• Simple one-step procedure; takes 1-2 hours

• Fast and convenient

• Comparison of the absorbance of pNA from an apoptotic sample with an
uninduced control allows determination of the fold increase in CPP32 activity.

Kit
components:

• Cell Lysis buffer

• 2X Reaction Buffer

• DEVD-pNA (4 mM)

• DTT (1 M)

• Dilution Buffer

Description:


Activation of ICE-family proteases/caspases initiates apoptosis in mammalian
cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and
convenient means for assaying the activity of caspases that recognize the
sequence DEVD. The assay is based on spectrophotometric detection of the
chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate
DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or
a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of
pNA from an apoptotic sample with an uninduced control allows determination of
the fold increase in CPP32 activity.

Storage
Conditions:

-20°C

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【e-mail】taiding.biotech@msa.hinet.net

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com