Caspase-3
Colorimetric Assay Kit





Caspase-3活性分析套組





Catalog#:
K106-100 | Size: 100 assays





Kit
Summary:


• Detection method- Absorbance (400 or 405 nm)

• Sample type- Cell and tissue lysates

• Species reactivity- Mammalian

• Kit size- Convenient sizes (25, 100, 200, 400 assays)

• Applications- Detect early/middle stages of apoptosis; differentiate
apoptosis from necrosis.





Features
& Benefits:


• Simple one-step procedure; takes 1-2 hours

• Fast and convenient

• Comparison of the absorbance of pNA from an apoptotic sample with an
uninduced control allows determination of the fold increase in CPP32 activity.





Kit
components:


• Cell Lysis buffer

• 2X Reaction Buffer

• DEVD-pNA (4 mM)

• DTT (1 M)

• Dilution Buffer





Description:


Activation of ICE-family proteases/caspases initiates apoptosis in mammalian
cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and
convenient means for assaying the activity of caspases that recognize the
sequence DEVD. The assay is based on spectrophotometric detection of the
chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate
DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or
a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of
pNA from an apoptotic sample with an uninduced control allows determination of
the fold increase in CPP32 activity.





Storage
Conditions:


-20°C





 




















































































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【供應廠商】太鼎生物科技有限公司



【連絡人】許虹宜 0920-312382



【e-mail】taiding.biotech@msa.hinet.net



【台北公司】02-86609496 【傳真】02-86609342



【相關網址】 www.biopioneer.com.tw



【原廠網址】 www.biovision.com





 


 

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