太鼎生物科技/太鼎食安科技

Live-Dead
Cell Staining Kit

Detects Live-Dead Cells by FL

Live-Dead Cell Staining Kits

Catalog#: K501-100 | Size: 100 staining

研究生長控制和細胞死亡，細胞活性和死亡，為重要之方法。BIOVISIOIN提供簡單之試劑，一個步驟分析細胞活性與死亡比例。可應用於螢光顯微鏡、螢光reader或是流式細胞技術，觀察活細胞與死細胞之比例。Live-Dye具有細胞滲透性，可被Live-Dye染劑染色經由Ex/Em =
488/518 nm螢光激發，活細胞呈現綠色。PI染劑染色，經由Ex/Em =
488/615 nm螢光激發，死細胞呈色紅色。

本試劑包裝為24well 100個分析。

Distinguishing
between live and dead cells is very important for investigation of growth
control and cell death. The Live-Dead Cell Staining Kit provides the
ready-to-use reagents for convenient discrimination between live and dead
cells. The kit utilizes Live-DyeTM, a cell-permeable green fluorescent dye
(Ex/Em = 488/518 nm), to stain live cells. Dead cells can be easily stained by
propidium iodide (PI), a cell non-permeable red fluorescent dye (Ex/Em =
488/615 nm). Stained live and dead cells can be visualized by fluorescence
microscopy using a band-pass filter (detects FITC and rhodamine). The kit
provides sufficient reagents for 100 stainings using 24-well plate.

Kit
Summary:

• Detection method- Fluorescent microscopy (Ex/Em 488/518 nm) to detect staines
live cells; (Ex/Em 488/615) to detect stained dead cells

• Sample type- Cell culture (adherent and suspension cells)

• Species reactivity- Mammalian

• Applications- Stained live and dead cells can easily be visualized by
fluorescence microscopy.

Features
& Benefits:

• Simple procedure; takes less than 20 minutes

• Fast and convenient

Kit
components:

• Solution A (1 mM Live-Dye)

• Solution B (2.5 mg/ml PI)

• Staining Buffer

Description:


Distinguishing between live and dead cells is very important for investigation
of growth control and cell death. The Live-Dead Cell Staining Kit provides the
ready-to-use reagents for convenient discrimination between live and dead
cells. The kit utilizes Live-Dye™, a cell-permeable green fluorescent dye
(Ex/Em = 488/518 nm), to stain live cells. Dead cells can be easily stained by
propidium iodide (PI), a cell non-permeable red fluorescent dye (Ex/Em =
488/615). Stained live and dead cells can be visualized by fluorescence
microscopy using a band-pass filter (detects FITC and rhodamine). The kit
provides sufficient reagents for 100 stainings using 24-well plate.

Storage
Conditions:

-20°C