太鼎生物科技/太鼎食安科技

Cat.No.:

Y051808

Quantity:

100μl

Antibody Type

Primary Antibody

Description

Anti-Escherichia coli Peroxidase Conjugate Goat is a This product
consists of purified IgG fraction of the above antiserum covalently coupled
to a highly purified preparation of horseradish peroxidase (RZ >3) by
methods . Care is taken to ensure adequate conjugation while preserving
maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration
in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is
2-3.NOTE: sodium azide is a potent inhibitor of peroxidase !

Immunogen

Mixture of E. coli serotypes

Species Reactivity

CROSS REACTIVITY: Antiserum is unabsorbed and does crossreact with
related Enterobacteriaceae UNINFECTED CELL REACTIVITY: Not applicable

Specificity

Many "O" and "K" antigenic serotypes of Escherichia
coli

Tested Applications

TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125,
O55, O111 & K12

Application Notes

This product is suitable for immunohistochemical, immunoblotting and EIA
applications. Working strength must be determined by the user but a starting
range of 1:20 - 1:200 is recommended for immunochemistry and blotting while
1:200 - 1:1,000 is recommended for for enzyme immunoassays.

Raised In

Goat

Clonality

Polyclonal

Isotype

IgG

Purity

purified IgG fraction

Storage Buffer

PBS, pH 7.4 with 0.05% sodium azide.

Formulation

Liquid

Concentration

1ug/ul

Storage

Recommended short term (<6 months) storage is liquid at 2-8°C. For
longer term storage, aliquot and freeze.

Caution

Anti-Escherichia coli Peroxidase Conjugate Goat is for research use only
and is not intended for therapeutic or diagnostic applications. Please
contact a technical service representative for more information.