【Influenza A H1N1 流感NP蛋白質】貨號#11675-V08B 。Nucleoprotein / NP Protein, NP蛋白質－太鼎生物科技/太鼎食安科技

【產品名稱】

Influenza
A H1N1 Nucleoprotein / NP Protein

【產品規格】

·
200 μg:

·
≥1mg Bulk:

【產品特點】

H1N1 NP / Nucleoprotein Protein
Product Information

Synonym :	NP
Protein Construction:	A DNA sequence encoding the Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein (B4URE0.1) (Met 1-Gly 490) was fused with a polyhistidine tag at the C-terminus.
Source:	Influenza A Virus H1N1
Expression Host:	Baculovirus-Insect cells

H1N1 NP / Nucleoprotein Protein QC
Testing

Purity:	> 97 % as determined by SDS-PAGE	SDS-PAGE: H1N1 NP protein
Endotoxin:	< 1.0 EU per μg of the protein as determined by the LAL method
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	Met 1
Molecular Mass:	The recombinant Influenza A virus ( A/Puerto Rico/8/34/Mount Sinai (H1N1) ) nucleoprotein comprises 501 amino acids and has a predicted molecular mass of 56.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions
Formulation:	Lyophilized from sterile 60mM Tris, 500mM NaCl , pH 7.4 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

【產品保存方法】

H1N1 NP / Nucleoprotein Protein Usage
Guide

Storage:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

【產品Datasheet】

11675-V08B

H1N1 NP / Nucleoprotein Protein
Description

Influenza (flu) is a respiratory infection in
mammals and birds. This virus is divided into three main types (A, B and C).
Influenza A is found in a wide variety of bird and mammal species. Influenza B
is largely confined to humans and is an important cause of morbidity. Influenza
C infects humans, dogs and pigs, sometimes causing both severe illness and
local epidemics. Influenza A is further divided into subtypes based on
differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA).
The notation HhNn is used to refer to the subtype comprising the hth discovered
HA protein and the nth discovered NA protein. Influenza viruses A, B and C are
very similar in overall structure. The viral particles of all influenza viruses
are similar in composition. These are made of a viral envelope containing two
main types of glycoproteins, wrapped around a central core. The central core
contains the viral RNA genome and other viral proteins that package and protect
this RNA. The influenza A genome contains 11 genes on eight pieces of RNA,
encoding for 11 proteins: Hemagglutinin (HA), Neuraminidase (NA), Nucleoprotein
(NP), M1, M2, NS1, NS2(NEP), PA, PB1, PB1-F2 and PB2.

Influenza A virus nucleoprotein (NP) forms homo-oligomers and multiple copies
of NP wrap around genomic RNA, along with a trimeric polymerase making up
ribonucleoprotein (RNP) complex. NP is composed of a head and a body domain and
a tail loop / linker region. The head domain is more conserved than the body
domain. NP oligomerization is mediated by the insertion of the non-polymorphic
and structurally conserved tail loop of one NP molecule to a groove of another
NP. The different form of NP oligomers is due to the flexibility of the
polymorphic linkers that join the tail loop to the rest of the protein. The RNA
binding property of NP is known to involve the protruding element and the
flexible basic loop between the head and body domains, both having high degree
of primary sequence conservation.