太鼎生物科技/太鼎食安科技

Hydroxyproline Assay Kit
Catalog#: K555-100  | Size: 100 assays

4-羥基脯胺酸 (4-hydroxyproline) 是脯胺酸的衍生物和 5-羥基離胺酸 (5-hydroxylysine)，二者可見於結締組織的膠原蛋白中 (collagen)。

Hydroxyproline (4-hydroxyproline, 4-羥基脯胺酸) is a common nonproteinogenic amino acid. It is found only in collagen and elastin in mammals but exists in a number of other proteins in plants. Hydroxyproline is formed only as a post-translational modification in the peptide chain and proline hydroxylase does not hydroxylate free proline. Hydroxyproline in tissue hydrolysates is a direct measure of the amount of collagen or gelatin present. A variety of disease states are believed to affect collagen turnover and can cause elevated serum or urine hydroxyproline. Such conditions range from neoplastic, inflammatory, renal or bone disease to endocrine and autoimmune disorders. BioVision’s Hydroxyproline Assay Kit is designed to measure hydroxyproline in tissue or protein/peptide hydrolysates. It can be used to measure hydroxyproline from other biological samples such as serum or urine if they have undergone a prior purification process. It is an easy, convenient method which results in a chromogen with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-2 μg.

Kit Summary:
• Detection method- Absorbance (560 nm)
• Sample type- Cell and Tissue  culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications-  The assay is useful over the range of 0.1-2 μg.

Features & Benefits:
• Simple  procedure; takes less than ~100 minutes
• Convenient and easy to use

Kit components:
• Oxidation Buffer
• Chloramine T Concentrate
• Perchloric acid/Isopropanol Solution
• DMAB Concentrate (in DMSO)
• Hydroxyproline Standard (1 mg/ml)