細胞遷移 | Cell Migration Assay Kit (24 well,8μM)貨號KTA5010

活動期間2022/10/01-2022/12/31 (來電洽詢0920312382許虹宜) 

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Cell Migration Assay Kit

產品描述:

細胞遷移(Cell migration)是指細胞受外界信號刺激,從一個地方遷移(migrate到)另一個地方的特性。另一種,通常發生在傷口癒合(wound healing)、細胞分化(cell differentiation)、胚胎發育(embryonic development)和腫瘤轉移(tumor metastasis等)過程中。Transwell 是一種實驗技術,可以在體外模擬人體的許多黏膜(mucosal)和生物屏障系統(biological barrier systems)。這該技術的主要材料是 Transwell 小室,Abbkine Cell Migration Assay Kit (24-Well Plate, 8 µm) 使用聚碳酸酯膜(polycarbonate membrane,  8 µm 孔徑)來測量細胞的遷移特性(migration characteristics)。原理是把Transwell將小室放入培養板中,小室稱為上室,培養板內部稱為下室腔室,上下層培養基由聚碳酸酯膜(polycarbonate membrane,  8 µm 孔徑)隔開。聚碳酸酯膜(polycarbonate membrane,  8 µm 孔徑)是可滲透的,下層培養基中的成分會影響上層的細胞。通過染色(staining)和洗脫(elution),計數細胞數以反映細胞的遷移能力(migration ability)。

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原廠操作下載

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Product name Cell Migration Assay Kit (24 well,8μM)
Applications notes Cell Migration Assay Kit (24 well,8μM) is used to detect cell migration with relative accuracy.
Kit components •24-Well Migration Plate
•Fixation Solution
•Staining Solution
•Elution Solution
Features & Benefits • Easy to operate.
• Simple, optimized experimental method.
• To detect the migration ability of cells.
Storage instructions Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

 

貨號 產品名稱 胞器染色位置 螢光波段  
貨號KTC4003 TraKine™粒線體染色 粒線體(mitochondria) Ex / Em = 490/523 nm 增殖(proliferating)和非增殖細胞(non-proliferating cells)
貨號KTC4100 Pro活細胞微管蛋白染色 微管蛋白(Tubulin) Ex/Em = 500/520 nm 活細胞或固定之細胞
貨號KTC4001 Abbkine 細胞膜染色  細胞膜 (plasma membrane) Ex/Em = 484/501 nm 活細胞、固定懸浮、貼附細胞
貨號BMD00063 DAPI Staining Solution 細胞核 dsDNA Ex/Em: 358/461 nm (with DNA)  染色活細胞和固定細胞
貨號KTC4210 活細胞Lysosome染色 Lysosome deep red fluorescent dye , Ex/Em:650/665nm  染色活細胞和固定細胞

 

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