太鼎生物科技/太鼎食安科技

Product name:

Zebrafish MDA(Malondialdehyde) ELISA Kit

Reactivity:

Fish

Alternative Names:

Malondialdehyde; MDA

Assay Type:

Competitive Inhibition

Sensitivity:

5.39 pg/mL

Standard:

1000 pg/mL

Range:

15.63-1000 pg/mL

Sample Type:

serum, plasma and other biological fluids

Assay Length:

2h

Research Area:

Metabolic pathway;Hepatology;Hormone metabolism;

Test principle:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Malondialdehyde(MDA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Malondialdehyde(MDA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Malondialdehyde(MDA) in the samples is then determined by comparing the OD of the samples to the standard curve.