太鼎生物科技/太鼎食安科技

Product name:

Zebrafish GSH(Glutathione) ELISA Kit

Reactivity:

Fish

Alternative Names:

L-Reduced Glutathione

Assay Type:

Competitive Inhibition

Sensitivity:

0.48 ng/mL

Standard:

100 ng/mL

Range:

1.57-100 ng/mL

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length:

2h

Research Area:

Metabolic pathway;

Test principle:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glutathione(GSH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione(GSH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione(GSH) in the samples is then determined by comparing the OD of the samples to the standard curve.