Mouse Anti-Human IgG Fc-UNLB (JDC-10)抗體 | 貨號9040-01規格0.5mg (0.5 mg/ml, 1ml)

 

產品介紹: 純化的抗人 IgG Fc 抗體(Purified Anti-Human IgG Fc antibody)，應用於 ELISA、流式細胞術(flow cytometry)、免疫組織化學(immunohistochemistry)、蛋白質印跡(western blot)和多重檢測(multiplex assays)。

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Clone JDC-10
Isotype Mouse IgG1κ
Isotype Control Mouse IgG1-UNLB (15H6)
Specificity Human/Rhesus/Chimpanzee IgG Fc
Immunogen Purified human IgG1 myeloma protein
Conjugate UNLB (Unconjugated)
Buffer Formulation Borate buffered saline, pH 8.2
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C
Applications ELISA – Quality tested 1-15,20
FLISA – Quality tested
Flow Cytometry – Quality tested 18,19
Immunohistochemistry-Paraffin Sections – Reported in literature 16
Western Blot – Reported in literature 17
Multiplex – Reported in literature 20-22
RRID Number AB_2796598

 

細胞免疫專家極力推薦SouthernBiotech高品質二級抗體

美國抗體生產商Southern Biotech於1982年，由UAB細胞免疫專家Dr. Max Cooper與實驗室成員共同創立。SouthernBiotech致力於生產高品質二級抗體。物種種類齊全，包括有Bovine、Canine、Chicken、Cynomolgus、Donkey、Equine、Feline、Goat、Guinea Pig、Hamster、Human、Monkey、Mouse、Porcine、Rabbit、Rat、Rhesus、Sheep、Turkey。具有螢光物質（FITC、PE、APC、SPRD、PE/CY5.5、APC/CY5.5、Spectral Red、Alexa 488、Alexa 647、Pacific Blue）各種螢光標誌、另具酵素（HRP、AP）、或未標誌及Low Endotoxin/Azide Free（LE/AF）等標定。

品牌商品繪圖 250x250 SBA _工作區域 1_工作區域 1_工作區域 1.jpg

SouthernBiotech 抗小鼠二抗(anti-mouse secondary antibodies)是經過廣泛表徵的親和純化(affinity-purified)的多克隆(polyclonal)或單克隆抗體(monoclonal antibodies)，對小鼠免疫球蛋白重鏈類別(mouse immunoglobulin heavy chain classes)和亞類(subclasses)、輕鍊(light chains)和片段(fragments)具有特異性(specificities)。SouthernBiotech提供在山羊(goat)、兔(rabbit)、驢(donkey)和大鼠(rat)中產生的抗小鼠二抗。它們在檢測一抗(primary antibodies )方面很有價值，並且有多種形式可供選擇，包括未偶聯以及酶、生物素化和螢光偶聯物，用於 ELISA、蛋白質印跡(western blot)、流式細胞術(flow cytometry)、多重和免疫組織化學(immunohistochemistry)等分析。

由於單克隆抗體 ( monoclonal antibodies )將結合抗體靶標上的單個表位，因此它們具有高度特異性(specific)，可提供出色的批次間一致性(lot-to-lot consistency)，並且通常在文獻中得到很好的表徵。我們的多克隆抗小鼠二抗(polyclonal anti-mouse secondary antibodies)可以通過信號放大提高靈敏度(sensitivity)，因為多個二抗可以與單個一抗結合。

選定的抗體已與來自其他物種的 IgG 和/或血清蛋白交叉吸附，以產生對這些物種具有最小交叉反應性(cross-reactivity)的抗體。使用對您的樣品物種具有最小交叉反應性的二抗非常重要。通過提供全面的多克隆(polyclonal)和單克隆(monoclonal)抗小鼠類(anti-mouse class)和亞類特異性二抗（例如 IgG1、IgG2b、IgM），可以在測定中同時檢測多種小鼠一抗。我們有廣泛的全分子和F(ab’)2 fragment 片 段多克隆和單克隆抗小鼠抗體可供選擇，所有這些抗體都是內部生產和驗證的，可用於您的特定檢測。

 

Isotype control 分型對照組 | 分型對照組套組 | 抗體分型ELISA 套組 | anti-rabbit 二級抗體 | anti-mouse二級抗體 |

 

  1. 1. Raux M, Finkielsztejn L, Salmon-Céron D, Bouchez H, Excler JL, Dulioust E, et al. IgG subclass distribution in serum and various mucosal fluids of HIV type 1-infected subjects. AIDS Res Hum Retroviruses. 2000;16:583-94. (ELISA)
  2. 2. Chapuy-Regaud S, Nogueira L, Clavel C, Sebbag M, Vincent C, Serre G. IgG subclass distribution of the rheumatoid arthritis-specific autoantibodies to citrullinated fibrin. Clin Exp Immunol. 2005;139:542-50. (ELISA)
  3. 3. Qian Y, Diaz LA, Ye J, Clarke SH. Dissecting the anti-desmoglein autoreactive B cell repertoire in pemphigus vulgaris patients. J Immunol. 2007;178:5982-90. (ELISA)
  4. 4. Adekar SP, Jones RM, Elias MD, Al-Saleem FH, Root MJ, Simpson LL, et al. Hybridoma populations enriched for affinity-matured human IgGs yield high-affinity antibodies specific for botulinum neurotoxins. J Immunol Methods. 2008;333:156-66. (ELISA)
  5. 5. Xue L, Johnson R, Gorovits B. Prevalence and isotypic complexity of the anti-Chinese hamster ovary host cell protein antibodies in normal human serum. AAPS J. 2010;12:98-106. (ELISA)
  6. 6. Dreskin SC, Tripputi MT, Aubrey MT, Mustafa SS, Atkins D, Leo HL, et al. Peanut-allergic subjects and their peanut-tolerant siblings have large differences in peanut-specific IgG that are independent of HLA class II. Clin Immunol. 2010;137:366-73. (ELISA)
  7. 7. de Smit M, Westra J, Vissink A, Doornbos-van der Meer B, Brouwer E, van Winkelhoff AJ. Periodontitis in established rheumatoid arthritis patients: a cross-sectional clinical, microbiological and serological study. Arthritis Res Ther. 2012;14:R222. (ELISA)
  8. 8. Geisler WM, Morrison SG, Doemland ML, Iqbal SM, Su J, Mancevski A, et al. Immunoglobulin-specific responses to Chlamydia elementary bodies in individuals with and at risk for genital chlamydial infection. J Infect Dis. 2012;206:1836-43. (ELISA)
  9. 9. Christianson GJ, Sun VZ, Akilesh S, Pesavento E, Proetzel G, Roopenian DC. Monoclonal antibodies directed against human FcRn and their applications. MAbs. 2012;4:208-16. (ELISA)
  10. 10. Ravn P, Madhurantakam C, Kunze S, Matthews E, Priest E, O'Brien S, et al. Structural and pharmacological characterization of novel potent and selective monoclonal antibody antagonists of glucose-dependent insulinotropic polypeptide receptor. J Biol Chem. 2013;288:19760-72. (ELISA)
  11. 11. Beaumont E, Patient R, Hourioux C, Dimier-Poisson I, Roingeard P. Chimeric hepatitis B virus/hepatitis C virus envelope proteins elicit broadly neutralizing antibodies and constitute a potential bivalent prophylactic vaccine. Hepatology. 2013;57:1303-13. (ELISA)
  12. 12. Dimitrov JD, Planchais C, Scheel T, Ohayon D, Mesnage S, Berek C, et al. A cryptic polyreactive antibody recognizes distinct clades of HIV-1 glycoprotein 120 by an identical binding mechanism. J Biol Chem. 2014;289:17767-79. (ELISA)
  13. 13. Einarsdottir HK, Stapleton NM, Scherjon S, Andersen JT, Rispens T, van der Schoot CE, et al. On the perplexingly low rate of transport of IgG2 across the human placenta. PLoS One. 2014;9(9):e108319. (ELISA)
  14. 14. Westra J, van Assen S, Wilting KR, Land J, Horst G, de Haan A, et al. Rituximab impairs immunoglobulin (Ig)M and IgG (subclass) responses after influenza vaccination in rheumatoid arthritis patients. Clin Exp Immunol. 2014;178:40-7. (ELISA)
  15. 15. Kapur R, Della Valle L, Verhagen OJ, Hipgrave Ederveen A, Ligthart P, de Haas M, et al. Prophylactic anti-D preparations display variable decreases in Fc-fucosylation of anti-D. Transfusion. 2014 Sep 19. doi: 10.1111/trf.12880. [Epub ahead of print]. (ELISA)
  16. 16. Pienaar IS, Lee CH, Elson JL, McGuinness L, Gentleman SM, Kalaria RN, et al. Deep-brain stimulation associates with improved microvascular integrity in the subthalamic nucleus in Parkinson's disease. Neurobiol Dis. 2015;74:392-405. (IHC-PS)
  17. 17. Lamoureux J, Aubin E, Lemieux R. Autoantibodies purified from therapeutic preparations of intravenous immunoglobulins (IVIg) induce the formation of autoimmune complexes in normal human serum: a role in the in vivo mechanisms of action of IVIg?. Int Immunol. 2004;16:929-36. (WB)
  18. 18. Leyendeckers H, Odendahl M, Löhndorf A, Irsch J, Spangfort M, Miltenyi S, et al. Correlation analysis between frequencies of circulating antigen-specific IgG-bearing memory B cells and serum titers of antigen-specific IgG. Eur J Immunol. 1999;29:1406-17. (FC)
  19. 19. Cortez VS, Cervantes-Barragan L, Song C, Gilfillan S, McDonald KG, Tussiwand R, et al. CRTAM controls residency of gut CD4+CD8+ T cells in the steady state and maintenance of gut CD4+ Th17 during parasitic infection. J Exp Med. 2014;211:623-33. (FC)
  20. 20. Dasso J, Lee J, Bach H, Mage RG. A comparison of ELISA and flow microsphere-based assays for quantification of immunoglobulins. J Immunol Methods. 2002;263:23-33. (ELISA, Multiplex)
  21. 21. Tomaras GD, Yates NL, Liu P, Qin L, Fouda GG, Chavez LL, et al. Initial B-cell responses to transmitted human immunodeficiency virus type 1: virion-binding immunoglobulin M (IgM) and IgG antibodies followed by plasma anti-gp41 antibodies with ineffective control of initial viremia. J Virol. 2008;82:12449-63. (Multiplex)
  22. 22. Goepfert PA, Elizaga ML, Seaton K, Tomaras GD, Montefiori DC, Sato A, et al. Specificity and 6-month durability of immune responses induced by DNA and recombinant modified vaccinia Ankara vaccines expressing HIV-1 virus-like particles. J Infect Dis. 2014;210:99-110. (Multiplex)
  23. 23. Nonhuman Primate Reagent Resource (Rhesus & Chimpanzee Reactivity)
 
 
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