HDAC
Substrate, Colorimetric

Catalog#: 2207-25 | Size: 25 mg

Product
Name: HDAC Substrate, Colorimetric

Alternate
Name/Synonyms: Boc- Lys(Ac)-pNA

Description:
A
colorimetric substrate for histone deacetylases (HDACs).

Peptide
Sequence: Boc- Lys(Ac)-pNA

Appearance:
Pale
yellow solid

Formulation:
N/A

CAS
Number: N/A

Molecular
Formula: C₁₉H₂₈N₄O₆

Molecular
Weight: 408.45

Purity:
≥98%
by HPLC

Solubility: DMSO

Storage
Temp.: -20°C

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

Elastase
Substrate, Fluorogenic

A fluorogenic substrate for neutrophil elastase

Catalog#: 1975-5 | Size: 5 mg

Alternate Name/Synonyms: MeOSuc - Ala - Ala - Pro - Val – AFC;
MeOSuc-AAPV-AFC

Peptide Sequence: N/A

Appearance: Lyophilized solid

Formulation: N/A

CAS Number: N/A

Molecular Formula: C₃₁H₃₈N₅O₉F₃

Molecular Weight: 681.67

Purity: ≥99% by HPLC

Solubility: DMSO

Storage Temp.: -20°C

Shipping Conditions: gel pack

Description: A useful fluorogenic substrate for
neutrophil elastase. Cleavage of this peptide by the enzyme generates a
strongly fluorescent AFC that is monitored fluorometrically. λex: 380 nm; λem:
500 nm.

Handling: Protect from light and moisture

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

Caspase
Colorimetric Substrate Set

Set of 7:
Caspase-1, 2, 3, 5, 6, 8 & 9



Catalog#:
K132-7-25 | Size: 7 x 25 assay

Kit
Summary:

• Detection method- Absorbance (400 or 405 nm )

• Sample type- Cell culture

• Species reactivity- Mammalian

• Kit size- 7X25 assays

• Applications- Detects specific Caspases involved in apoptosis within cells

Features
& Benefits:

• Contains ready-to-use caspase-1/-2/-3/-5/-6/-8/-9 pNA substrates

Kit
components:

• Caspase-1 Substrate, Ac-YVAD-pNA

• Caspase-2 Substrate, Ac-VDVAD-pNA

• Caspase-3 Substrate, Ac-DEVD-pNA

• Caspase-5 Substrate, Ac-WEHD-pNA

• Caspase-6 Substrate, Ac-VEID-pNA

• Caspase-8 Substrate, Ac-IETD-pNA

• Caspase-9 Substrate, Ac-LEHD-pNA

Description:


Ready-to-use colorimetric substrates for members of caspase family proteases.
All substrates were provided in liquid ready-to-use form.

Storage
Conditions:

-20°C

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

CaspGLOW
Fluorescein Active Caspase Staining Kit

Catalog#: K180-100 | Size: 100 assays

Kit Summary:

• Detection method- Fluorescence microscopy, Flow cytometry and fluorescence
plate reader (Ex. = 485 nm and Em. = 535 nm)

• Sample type- Live cells

• Species reactivity- Mammalian

• Kit size- Convenient sizes (25 and 100 assays)

• Applications- Sensitive detection of activated caspases in living cells.

Features & Benefits:

• Simple one-step procedure; takes only 1-2 hours

• Fast and convenient

• The FITC label allows for direct detection of the activated caspases in
apoptotic cells

Kit components:

• FITC-VAD-FMK

• Wash Buffer

• Z-VAD-FMK

Description:

Activation of caspases plays a central role in apoptosis. The CaspGLOW™
Fluorescein Active Caspase Staining Kit provides a convenient and sensitive
means for detecting activated caspases in living cells. The assay utilizes the
caspase family inhibitor VAD-FMK conjugated to FITC (FITC-VAD-FMK) as a marker.
FITC-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated
caspases in apoptotic cells.

Storage
Conditions:

-20°C

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

Yeast
Mitochondria Isolation Kit

Catalog#: K259-50 | Size: 50 assays

Assay Kit Name:

Yeast Mitochondria Isolation Kit

Kit Summary:

Application:

• Isolation of highly pure intact mitochondria from yeast cells.

• Mitochondrial respiration studies, assembly of the complexes, apoptosis,
mtDNA and mtRNA, and for protein profiling.

• Western blot and ELISA

Sample Type:

• Yeast cells culture.

Features & Benefits:

• Simple & convenient protocol

• The kit provides unique formulations of ready-to-use buffers and reagents to
isolate intact mitochondria

Kit Components:

• Buffer A

• Buffer B

• 1 M DTT

• Homogenization Buffer

• Lysis Enzyme Mix

• Storage Buffer

• Protease Inhibitor Cocktail

Description:

Mitochondria are the power house of the cells because they generate most of the
supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are
double membrane organelles: an outer membrane and a folded inner membrane
called cristae. Isolated mitochondria is a useful tool to study mitochondrial
respiration, assembly of the respiratory complexes, apoptosis, mtDNA and mtRNA,
and for protein profiling. BioVision’s Yeast Mitochondria Isolation kit will
enable fast and easy purification of mitochondria from yeast cells, utilizing
yeast cell wall lysis and homogenization.

This Mitochondria Isolation Kit is tested for Pichia pastoris &
Saccharomyces cerevisiae & can be used to isolate mitochondria from other
yeast. Under fermentable media, the yield is ~150-200 µg of mitochondria &
under non-fermentable media (e.g. Glycerol) ~200-250 µg of mitochondria from a
culture of OD ~20.

Storage Conditions:

-20°C

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

Apoptotic DNA Ladder Assays

BioVision offers
three novel Apoptotic DNA Ladder Detection Kits. Quick Apoptotic DNA Ladder
Detection Kit offers fast detection of DNA fragmentation in apoptotic cells. Unlike other
similar kits on the market which require 1-2 days to prepare nucleic acids, the
new procedure requires less than 90 minutes to prepare chromosomal DNA in a
single tube. The new procedure increases recovery of small fragmented DNA, and
therefore improves detection sensitivity. Enhanced Apoptotic DNA Ladder
Detection Kit incorporates a proprietary buffer system and a highly sensitive
staining dye, which dramatically increases the sensitivity of detection. The
newly released Apoptotic DNA ladder Isolation Kit can specifically extract DNA
ladder, giving the highest sensitivity. The kit can detect apoptotic DNA ladder
from both tissue and cells with as little as 5% apoptotic cell population. DNA
ladders can easily be visualized by agarose gel electrophoresis.

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

Mitochondrial
Protein IP Kit

Catalog#: K285-50 | Size: 50 assays

Assay Kit Name:

Mitochondrial Protein IP Kit

Kit Summary:

Application:

• Optimized for compatibility with immunoprecipitation (IP and co-IP) and
pull-down using tagged proteins.

• Gentle formulation for maintenance of stable mitochondrial complexes.

• Compatible with SDS PAGE, 2D gel, Blue-Native gel, Mass spectrometry.

• Functional Assays and enzymatic assays.

Sample Type:

• Mitochondria, mitochondrial extract or cell lysate.

Features & Benefits:

• Simple & convenient protocol

• The Kit provides unique formulation of ready-to-use buffers & reagents
for IP/Co-IP/Pull down of mitochondrial proteins or complexes.

Kit Components:

• Mitochondria Protein IP Buffer

• Wash Buffer (5X)

• Protease Inhibitor Cocktail

• 10% n-Dodecyl-beta-D-maltoside

• 10% Triton X-100

• 10% Digitonin

Description:

Mitochondria are the power house of the cells and play an essential role in
energy production. Damage to the mitochondria activates signaling pathways that
induce apoptosis. Mitochondria also regulate and mediate transport of the
metabolites and ions needed for oxidative phosphorylation and maintenance of
membrane potential for ATP synthesis. Mitochondrial dysfunction leads to
several disorders like cardiac dysfunction, diabetes, aging and neurological
disorder, mainly caused by mutations in mitochondrial DNA or in nuclear genes
that code for mitochondrial components. Thus, mitochondria have several
different functions in the cell.

BioVision ready to use mitochondria Protein IP Buffer is optimized for
immunoprecipitation (IP and co-IP) using mitochondria and mitochondrial
extracts. The buffer is a gentle formulation, which maintains the stability of
mitochondrial complexes. The Mitochondrial Protein IP kit is provided with
different choices of detergents like n-Dodecyl-beta-D-maltoside, Triton X-100
and digitonin to achieve different stringency conditions for protein-protein
interaction studies. Triton X-100 is the most commonly used detergent
especially for membrane protein solubilization. However, in case of fragile
complexes digitonin or n-Dodecyl-beta-D-maltoside is the choice of detergents.

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

Yeast
Mitochondria (Pichia pastoris)

Catalog#:
1111-50 | Size: 50 μg

Product Highlights:

Applications:

• Mitochondrial respiration studies, assembly of the complexes, apoptosis,
mtDNA and mtRNA, and for protein profiling

• Western blot and ELISA

Description: Mitochondria are the power
house of the cells as they generate most of the supply of energy in the form of
adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles
comprising of an outer membrane and a folded inner membrane called cristae.
Isolated mitochondria are a useful tool to study mitochondrial respiration,
assembly of the respiratory complexes, apoptosis, mtDNA, mtRNA, protein
profiling, and as a loading control. Highly pure yeast mitochondria are
purified using standard protocol as mentioned in the Yeast Mitochondria
Isolation Kit - Cat # K259-100 from Pichia pastoris.

Storage
Temp.: -70°C

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

Ready-to-Use Apoptosis Inducers & Sets

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

Cell Cycle Analysis Kit

GenScript's Cell Cycle Analysis Kit enables rapid and convenient cell cycle and cell proliferation assay. In normal cells, DNA density changes depending on whether the cell is growing, dividing, resting, or performing its ordinary functions. Staining DNA using flow cytometry allows the researcher to determine whether the cell is in G0/G1, S, or G2/M, giving a clearer picture of how cells in different parts of the cell cycle are distributed throughout the sample and the level of proliferation activity. In apoptotic cells, DNA is degraded by endogenous nucleases and diffused out of cells. A highly definable sub-G1 peak occurs and is easily quantified by dyes. Cells can then be sorted and further analyzed by microscopy to verify the presence of apoptotic cells. After RNA is degraded by RNase, the nucleic acid dye in this kit binds with DNA in the nucleus, not only in normal cells, but also in apoptotic cells. The change in DNA of cells can be assayed by flow cytometry.

Key Features: Ready-to-use Format Enhanced Versatility: Directly analyzes cell cycle – not only suspension cells but also adherence cells. Superior Utility: Direct quantification of normal, apoptotic, and dead cells by flow cytometry.

Examples: Observation and analysis of the distribution of cell cycle by Cell Cycle Analysis Kit (L00287): P388 cells were induced apoptosis by 10 μM camptothecin for 1 hour at 37°C. The result is as follows:   訂購貨號 Information: L00287 Cell Cycle Analysis Kit   【供應廠商】太鼎生物科技有限公司 【連絡人】許虹宜 0920-312382 【台北公司】02-86609496 【傳真】02-86609342 【相關網址】 www.biopioneer.com.tw 【原廠網址】 www.genescript.com

Product Name: WST-8

Alternate Name/Synonyms: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monosodium salt

Description: A next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH. It is reduced by NADH at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nM. Useful in cell proliferation assays as a cell viability indicator.

Peptide Sequence: N/A

Appearance: Pale brown solid

Formulation: N/A

CAS Number: 193149-74-5

Molecular Formula: C₂₀H₁₃N₆O₁₁S₂Na

Molecular Weight: 600.47

Purity: ≥97% by HPLC

Solubility: Water (100 mM)

Storage Temp.: -20°

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496 【傳真】02-86609342

【相關網址】 www.biopioneer.com.tw

【原廠網址】 www.biovision.com

Jurkat
Cell Extract (Induced)

Jurkat Cell Extract (Induced)

Catalog#: 1107-1000 | Size: 1 mg

Product Highlights: CONCENTRATION-
4 µg/µl

Description: The cytosolic extract was prepared
from Jurkat cells induced with 2 µM camptothecin. The extract can be used as a
positive control for caspase activity assays or for Western blotting. We
recommend using 100-200 µg per assay for caspase activity assays and 10-20 µg
per assay for Western blotting.

Storage Temp.: -70°C

Shipping: EA

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496【傳真】02-86609342

【相關網址】www.biopioneer.com.tw

【原廠網址】www.biovision.com

Caspase-12
Inhibitor Z-ATAD-FMK

Caspase-12 Inhibitor Z-ATAD-FMK

Catalog#: 1079-100 | Size: 100 µl (2 mM)

Alternate
Name/Synonyms: Z-ATAD-FMK, Caspase-12 Inhibitor (fluoromethylketone)

Peptide
sequence: Z-Ala-Thr-Ala-Asp(OMe)-FMK

Appearance:
Liquid

Formulation:
DMSO
(2 mM)

Molecular
Formula: C₂₄H₃₃FN₄O₉

Molecular
Weight: 540.54

Purity:
>95%
by HPLC

Solubility:
N/A

Storage
Temp.: -20°C

Shipping
Conditions: gel pack

Description:
A
synthetic peptide that irreversibly inhibits caspase-12 and related caspase
activity. The inhibitor is designed as a methyl ester to facilitate cell
permeability. (CAUTION: If the intended use is on purified or recombinant
enzymes, esterase should be added to generate free carboxyl groups).

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496【傳真】02-86609342

【相關網址】www.biopioneer.com.tw

【原廠網址】www.biovision.com

Caspase-9
Substrate LEHD-AFC

Caspase-9 Substrate LEHD-AFC

Catalog#: 1075-1000 | Size: 1000 assays

Alternate Name/Synonyms: Ac-LEHD-AFC,
Caspase-9 Substrate, Fluorogenic

Peptide sequence: Ac-Leu-Glu-His-Asp-AFC

Appearance: Liquid

Formulation: DMSO ( 1 mM)

CAS Number: N/A

Molecular Formula: C₃₃H₃₈F₃N₇O₁₁

Molecular Weight: 765.7

Purity: >98% by HPLC

Solubility: N/A

Storage Temp.: -20°C

Shipping Conditions: gel pack

Description: Ready-to-use fluorometric substrate
for caspase-9/Mch-6 and related caspases that recognize the amino acid sequence
LEHD. Caspase activity can be quantified by fluorescent detection of free AFC
after cleavage from the peptide substrate LEHD-AFC at Ex. = 400 nm and Em. =
505 nm, using a fluorometer or multi-well fluorescence plate reader.
Alternatively, a shift in fluorescence from blue to green upon cleavage can be
visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate
provides an economic alternative for researchers who perform large amount of
caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer
(Cat. #1068-20, -80) and DTT (Cat.# 1201-1) for caspase assays are also
available separately.

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496【傳真】02-86609342

【相關網址】www.biopioneer.com.tw

【原廠網址】www.biovision.com

Caspase-9
Inhibitor Z-LEHD-FMK

Caspase-9 Inhibitor Z-LEHD-FMK

Catalog#: 1074-100 | Size: 100 µl (2 mM)

Alternate
Name/Synonyms: Z-LEHD-FMK, Caspase-9 Inhibitor (fluoromethylketone)

Peptide
sequence: Z-Leu-Glu(OMe)-His-Asp(OMe)-FMK

Appearance:
Liquid

Formulation:
DMSO
( 2 mM)

CAS
Number: N/A

Molecular
Formula: C₃₂H₄₃FN₆O₁₀

Molecular
Weight: 690.7

Purity:
>98%
by HPLC

Solubility:
N/A

Storage
Temp.: -20°C

Shipping
Conditions: gel pack

Description:
A
synthetic peptide that irreversibly inhibits caspase-9 and related caspase
activity. The inhibitor is designed as a methyl ester to facilitate cell
permeability. (CAUTION: If the intended use is on purified or recombinant
enzymes, esterase should be added to generate free carboxyl groups).

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496【傳真】02-86609342

【相關網址】www.biopioneer.com.tw

Caspase-2
Inhibitor Z-VDVAD-FMK

Caspase-2 Inhibitor Z-VDVAD-FMK

Catalog#: 1073-20C | Size: 20 µl (10 mM)

Alternate
Name/Synonyms: Z-VDVAD-FMK, Caspase-2 Inhibitor (fluoromethylketone)

Peptide
sequence: Z-Val-Asp(OMe)-Val-Ala-Asp(OMe)-FMK

Appearance:
Liquid

Formulation:
DMSO
( 10 mM)

CAS
Number: N/A

Molecular
Formula: C₃₂H₄₆FN₅O₁₁

Molecular
Weight: 695.7

Purity:
>98%
by HPLC

Solubility:
N/A

Storage
Temp.: -20°C

Shipping
Conditions: gel pack

Description:
A
synthetic peptide that irreversibly inhibits caspase-2 and related caspase
activity. The inhibitor is designed as a methyl ester to facilitate cell
permeability. (CAUTION: If the intended use is on purified or recombinant
enzymes, esterase should be added to generate free carboxyl groups).

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496【傳真】02-86609342

【相關網址】www.biopioneer.com.tw

【原廠網址】www.biovision.com

Caspase-8
Inhibitor Z-IETD-FMK

Caspase-8 Inhibitor Z-IETD-FMK

Catalog#: 1064-100 | Size: 100 µl (2 mM)

Alternate
Name/Synonyms: Z-IETD-FMK, Caspase-8 Inhibitor (fluoromethylketone)

Peptide
sequence: Z-Ile-Glu(OMe)-Thr-Asp(OMe)-FMK

Appearance:
Liquid

Formulation:
DMSO
(2 mM)

CAS
Number: N/A

Molecular
Formula: C₃₀H₄₃FN₄O₁₁

Molecular
Weight: 654.7

Purity:
>99%
by HPLC

Solubility:
N/A

Storage
Temp.: -20°C

Shipping
Conditions: gel pack

Description:
A
synthetic peptide that irreversibly inhibits FLICE and related protease/caspase
activity and blocks apoptosis. IETD is the specific recognition sequence found
in CPP32 proenzyme. The inhibitor is designed as a methyl ester to facilitate
cell permeability. (CAUTION: If the intended use is on purified or recombinant
enzymes, esterase should be added to generate free carboxyl groups.)

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496【傳真】02-86609342

【相關網址】www.biopioneer.com.tw

Caspase-8
Substrate IETD-AFC

Caspase-8 Substrate IETD-AFC

Catalog#: 1062-1000 | Size: 1000 assays

Alternate Name/Synonyms: Ac-IETD-AFC,
Caspase-8 Substrate, Fluorogenic

Peptide sequence: Ac-Ile-Glu-Thr-Asp-AFC

Appearance: Liquid

Formulation: DMSO (2 mM)

CAS Number: N/A

Molecular Formula: C₃₁H₃₈F₃N₅O₁₂

Molecular Weight: 729.7

Purity: >95% by HPLC

Solubility: N/A

Storage Temp.: -20°C

Shipping Conditions: gel pack

Description: Ready-to-use fluorometric substrate
for FLICE/caspase-8 and related caspases that recognize the amino acid sequence
IETD. The sequence IETD is based on caspase-8 cleavage site in CPP32/caspase-3
proenzyme. FLICE and related caspase activity can be quantified by fluorescent
detection of free AFC after cleavage from the peptide substrate IETD-AFC at Ex.
= 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate
reader. Alternatively, a shift in fluorescence from blue to green upon cleavage
can be visualized using a hand-held long-UV lamp.

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496【傳真】02-86609342

【相關網址】www.biopioneer.com.tw

【原廠網址】www.biovision.com