太鼎生物科技/太鼎食安科技

螢光細胞增生分析套組, BIOVISION K303-500

VisionBlue™ Quick Cell Viability Assay
Catalog#: K303-500  | Size: 500 assays

WWW.BIOVISION.COM

螢光細胞增生分析套組, BIOVISION K303-500

VisionBlue™ Quick Cell Viability Assay
Catalog#: K303-500  | Size: 500 assays

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安全性佳, 除了測量細胞增殖分析外, 可以再進行下游分析

Very safe: Non-radioactive, non-toxic, and cells can be further used for other experiments..

Kit Summary:
• Detection method- Fluorescence (Ex/Em 530–570/ 590-620 nm)
• Sample type- Cell culture (adherent and suspension)
• Species reactivity- Mammalian
• Kit size- Convenient sizes (500, 2500)
• Applications- This fluorescence-based single-step cell viabilty assay utilizes the redox dye (Resazurin) which is not fluorescent, but upon reduction by metabolically active cells, it becomes highly fluorescent (Ex = 530-570 nm; Em = 590 -620 nm). Therefore, viable metabolically active cells can be easily measured.

Features & Benefits:
• Simple one-step procedure
• Fast and convenient
• The assay is more sensitive than other similar assays (eg, Almar Blue reagent) on the market

Kit components:
• VisionBlue™ Reagent

Description:
The VisionBlue™ Quick Cell Proliferation Assay Kit provides by far the most sensitive and easiest means for quantifying cell proliferation and viability. Simply add the single reagent to cell culture, incubate and read the fluorescence intensity. The VisionBlue™assay utilizes the redox dye resazurin which is not fluorescent, but upon reduction by viable metabolically active cells, the dye becomes highly fluorescent (Ex = 530-570 nm; Em = 590-620 nm). Therefore, viable metabolically active cells can be easily measured.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans

The Quick Cell Proliferation Assay Kit provides all reagents and detailed instructions for a fast and sensitive quantification of cell proliferation and viability. The assay is based on the cleavage of the tetrazolium salt WST-1 to formazan by cellular mitochondrial dehydrogenases. Expansion in the number of viable cells resulted in an increase in the activity of the mitochondrial dehydrogenases, which leads to the increase in the amount of formazan dye formed. The formazan dye produced by viable cells can be quantified by multi-well spectrophotometer (microtiter plate reader) by measuring the absorbance of the dye solution at 440 nm. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds. The new method is so simple, requiring no washing, no harvesting, and no solubilization steps, and is faster and more sensitive than MTT, XTT, or MTSbased assays. The entire assay can be performed in a microtiter plate.

Calcein AM螢光染劑如何使用【太鼎生物科技有限公司02-86609496】

50ug的calcein AM可以溶在50ul的DMSO 最後濃度為1mM

取1ul的Calcein AM 溶在1X 的1000ul dissociation solution =1uM (1000ul)以96well 為例, 加入100ul的1Um Calcein AM,

大約可以使用500次.

詳細產品資訊，請登錄www.trevigen.com 查詢，或與太鼎生物科技有限公司(02)86609496聯繫。 

更多參考內容:

WWW.BIOPIONEER.COM.TW

 太鼎生物科技有限公司(02)86609496

更多參考內容:

原廠連結: http://www.biovision.com/

WWW.BIOPIONEER.COM.TW

 太鼎生物科技有限公司(02)86609496

最新消息!! 太鼎食安科技(bpfood)-成立新網站

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活細胞螢光染劑 Calcein AM，用於活細胞觀察及細胞侵入定量實驗, 1755-250

Catalog#: 1755-250  | Size: 5 x 50 µg

活細胞染劑 “Calcein AM”, 用於活細胞觀察及細胞侵入及轉移試劑.

配製方法:

50ug的calcein AM可以溶在50ul的DMSO 最後濃度為1Mm

取1ul的Calcein AM 溶在1X 的1000ul dissociation solution =1uM (1000ul)以96well 為例, 加入100ul的1Um Calcein AM,大約可以使用500次.

Calcein acetoxymethylester (Calcein AM) is a non-fluorescent, cell permeate compound that is hydrolyzed by intracellular esterases into the fluorescent anion calcein.

BIOVISION and TREVIGEN™ Calcein AM Fluorescent Dye can be used to fluorescently label viable cells for a variety of assays such as tumor cell invasion, endothelial cell migration, and endothelial cell tubulogenesis.

Alternate Name/Synonyms: Glycine,N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,bis[(acetyloxy)methyl] ester; Calcein acetoxymethyl ester

Peptide sequence: N/A

Appearance: Off-white solid

Formulation: N/A

CAS Number: 148504-34-1

Molecular Formula: C₄₆H₄₆N₂O₂₃

Molecular Weight: 994.86

Purity: ≥95% by HPLC

Solubility: DMSO (5 mg/ml)

Storage Temp.: -20°C

Shipping Conditions: gel pack

Description: A cell-permeable, non-fluorescent acetoxymethyl ester derivative of Calcein that gets hydrolyzed inside the cell by esterases. The loss of the acetoxymethyl group also enables calcein to readily bind intracellular calcium resulting in a strong yellowish-green fluorescence. It is the best probe for cell viablilty and cell adhesion due to its greater retention in cells as well and its pH insensitivity in physiological range.

Handling: Protect from air and light

USAGE: For Research Use Only! Not For Use in Humans.

更多參考內容:

原廠連結: http://www.trevigen.com/

WWW.BIOPIONEER.COM.TW

太鼎生物科技有限公司(02)86609496