CometAssay®


The single cell gel electrophoresis or CometAssay® is a state-of-the-art technique for quantitating DNA damage and repair from in vivo and in vitro samples of eukaryotic cells and some prokaryotic cells. In conjunction with Trevigen's new CometAssay® Electrophoresis System, which eliminates known causes of assay variability, it is the only technique that directly measures DNA damage in individual cells and as a result has rapidly gained importance in the fields of genetic toxicology and human biomonitoring.


cometseries


 



  1.  Cells mixed with low melting agarose at 37oC (LM Agarose)

  2.  Immobilize cells on CometSlide™

  3.  Treat cells with Lysis Solution (removes membranes and histones from the DNA)

  4.  Samples treated with alkali (unwinds and denatures DNA)

  5.  Samples stained with intercalating dye and visualized by epifluorescence microscopy following alkaline electrophoresis, which reveals DNA breaks.






















CometAssay® Reagents

Catalog #Product NameSize
4250-050-01CometAssay® Lysis Solution2 x 500 ml
4250-050-02CometAssay® LM Agarose15 ml
4250-050-05SYBR Green5 μl






















CometAssay® Reagent Starter Kits

Catalog #Product NameSize
4250-050-KCometAssay® Kit 50 Samples ( 25X2 well slides)50 samples
4252-040-KCometAssay® Kit, 40 Samples (2X20 well slides)40 samples
4253-096-KCometAssay® Kit 96 Samples (1X96)96 samples


















Silver Staining Kit

Catalog #Product NameSize
4251-050-KCometAssay® Silver (CommetAssay Kit w/silver staining reagents)50 samples
4254-200-KCometAssay® Silver Staining Kit200 samples






































CometAssay® Electrophoresis Systems

Catalog #Product NameSize
4250-050-ESCometAssay® Electrophoresis System1 system
4250-050-ESKCometAssay® 2 Well ES Unit w/ Starter Kit1 system, 1 starter kit
4252-040-ESKCometAssay® 20 Well ES Unit w/ Starter Kit1 system,1 starter kit
4252-040-ESK-PS1CometAssay® 20 Well ES Unit w/ Starter Kit plus Power Supply for North America1 system, 1 starter kit, 1 power supply
4253-096-ESKCometAssay® 96 Well ES Unit w/ Starter Kit1 system, 1 stater kit
4253-096-ESK-PS1CometAssay® 96 Well ES Unit w/ Starter Kit plus Power Supply for North America1 system, 1 starter kit, 1 power supply
4250-050-ESK-PS1CometAssay® 2 Well ES Unit w/ Starter Kit and Power Supply1 system, 1 starter kit, 1 power supply


















CometAsssay®Control Cells

Catalog #Product NameSize
4256-010-CCCometAssay® Alkaline Control Cells10 assays
4257-010-NCCometAssay® Neutral Control Cells10 assays






















































Slides and Rack System

Catalog #Product NameSize
3950-075-02FLARE™ Slides (3 Well, 25 Slides)25 slides
3950-300-02FLARE™ Slides (3 Well, 100 Slides)100 slides
4250-004-03CometSlide™ (2 Well, 2 Slides)2 slides
4250-050-03CometSlides™ (2 Well, 25, Slides)25 each
4250-200-03CometSlides™ (2 Well, 100 Slides)100 slides
4252-02K-01CometAssay® HT Slides (20 Well, 100 Slides)100 slides
4252-040-01CometAssay® HT Slides (20 Well, 2 Slides)2 slides
4252-040-02CometSlide™ Rack Systemeach
4252-200-01CometAssay® HT (20 Well, 10 Slides)10 slides
4252-500-01CometAssay® HT (20 Well, 25 Slides)25 slides
4253-096-03CometSlide™ (96 Well)1 slide






























































FLARE™ Assay Kits and Modules

Catalog #Product NameSize
3950-075-SPFLARE™ Sample Prep75 tests
4040-100-FKFpg FLARE™ Assay Kit75 samples
4040-100-FMFpg FLARE™ Module>100 samples
4045-01K-FKE. coli Endonuclease III FLARE™ Kit>75 samples
4045-01K-FME. coli Endonuclease III FLARE™ Module>100 samples
4055-100-FKT4-PDG FLARE™ Assay Kit75 samples
4055-100-FMT4-PDG FLARE™ Module>100 samples
4065-100-FKcv-PDG FLARE™ Assay Kit75 samples
4065-100-FMcv-PDG FLARE™ Module>100 samples
4100-100-FKUVDE FLARE™ Assay Kit75 samples
4100-100-FMUVDE FLARE™ Module>100 samples
4130-100-FKhOGG1 FLARE™ Assay Kit75 samples
4130-100-FMhOGG1 FLARE™ Module100 samples
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