ECL冷光試劑 | SuperKine™ West Femto Maximum Sensitivity Substrate貨號BMU102-EN

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SuperKine™ West Femto Maximum Sensitivity Substrate 提供了一種高度敏感的顯色溶液,可增強免疫學實驗的化學信號。為了獲得最佳的實驗結果,您需要優化所有實驗因素,包括樣品數量、抗體濃度(antibody concentration)、膜的使用和封閉劑 (blocking agents)。ECL 底物系列(ECL substrates)是一系列預混合(premixed)的即用型(ready-to-use)化學發光試劑(chemiluminescent reagents),用於檢測基於 HRP 的蛋白質(HRP-based Westerns)。 SuperKine™ West Femto 最大靈敏度底物專門針對免疫印跡上(picogram amounts) 的抗原檢測進行了優化。底物的強信號輸出和持續時間可以用照相或其他成像方法完美地檢測(picogram amounts)的抗原。

Fig. The sample is Mouse TNF-alpha protein (PRP1113, 17KD), the primary antibody is TNF-α Polyclonal Antibody (ABP0127, 1:2500), and the secondary antibody is HRP, Goat Anti-rabbit IgG (A21020, 1:10000). The exposure time is 30s.

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Product name

SuperKine™ West Femto Maximum Sensitivity Substrate

Kit components

• ECL HRP Substrate Reagent A
• ECL HRP Substrate Reagent B

Features & Benefits

The SuperKine™ West Femto Maximum Sensitivity Substrate developed by Abbkine is based on the innovation and optimization of the classic peroxidase substrate system. It is used for enhanced chemiluminescence (ECL) and can directly replace other expensive ECL products without the need to renew Optimize experimental conditions.

Usage notes

• Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution.
• Suggest 0.1-0.2ml of SuperLumia ECL working solution for 1 cm2 size of blot membrane.
• Incubate blot 1-5 minutes in SuperLumia ECL Substrate working solution.
• Drain excess reagent. Cover blot with clear plastic wrap.
• Expose blot to X-ray film.

Storage instructions

Stable for two years at 4-8°C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution.

Shipping

Gel pack with blue ice.

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活細胞和死細胞雙染色套組  |  CCK-8細胞增殖和細胞毒性試劑盒  | LDH細胞毒性測定試劑盒 | 細胞衰老檢測試劑盒(β-半乳糖苷酶 | 細胞增殖EdU Image試劑盒 

產品貨號

產品品項

反應

測量儀器

貨號KTA1001

活細胞和死細胞雙染色試劑盒

100個反應

活細胞綠色螢光染劑Ex/Em = 488/530 nm;

死細胞紅色螢光染料(Ex/Em = 535/617) 

貨號 KTA2010

 

TUNEL細胞凋亡檢測試劑盒

 

50個反應

螢光酶標儀、螢光顯微鏡或流式細胞儀。FITC通道(Ex / Em = 490nm / 520 nm)

貨號 KTC011001

 

CCK-8細胞增殖和細胞毒性試劑盒

500個反應

吸收光450nm

貨號KTC4003

TraKine™粒線體染色

50個反應/250個反應

Ex / Em = 490/523 nm增殖(proliferating)和非增殖細胞(non-proliferating cells)

貨號KTC4100

Pro活細胞微管蛋白染色

50個反應/250個反應

Ex/Em = 500/520 nm

貨號KTC4001

Abbkine 細胞膜染色 

100個反應/500個反應

Ex/Em = 484/501 nm

貨號BMD00063

DAPI Staining Solution

10MG/100MG

Ex/Em: 358/461 nm (with DNA)

貨號KTC4210

活細胞Lysosome染色

50個反應/250個反應

deep red fluorescent dye , Ex/Em:650/665nm

貨號KTA3030

細胞衰老檢測試劑盒(β-半乳糖苷酶)

100個反應

光學顯微鏡

貨號KTA1020 

CCK-8細胞增殖和細胞毒性試劑盒

1000個反應/10,000個反應

吸收光450nm

貨號KTA2030

細胞增殖EdU Image試劑盒

(BrdU替代法)

100個反應/500個反應

AbFluor 488 azide (Ex/Em = 501/525 nm)

貨號KTB1400

一氧化Dan(NO)檢測試劑盒 

96個反應/

192個反應

colorimetric (540 nm)

檢測範圍:1-100 µM

貨號KTB1070

CheKine™黃嘌呤氧化酶檢測

96個反應/

480個反應

OD 450 nm

線性範圍15.6 -500 mU/

貨號KTD3001

 BCA蛋白定量試劑盒

2000個反應/

5000個反應

OD 562 nm

相容性 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80

50 to 1000 µg/ml.

貨號KTB1110

乳酸脫氫酶(LDH)測定試劑盒

96個反應/

480個反應

λmax= 450 nm

1- 20 U/mL of LDH 

貨號PRP100153

IGF-I重組蛋白

200UG/1MG

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)

> 95 % as determined by SDS-PAGE

貨號 KTA2020

細胞週期染色檢測

100個反應/500個反應

Ex/Em=535/615 nm.

貨號BMC21400

PurKine™內毒素去除預裝離心柱

1ml×5

3mlx5

More than 2,000,000 EU/mL to eliminate >99% of endotoxins.

貨號KTA1200

CheKine™蛋白羰基比色測定試劑盒 

48個反應/

96個反應

吸收光370 nm

貨號KTB1080

超氧陰離子清除測定試劑盒

48個反應/

96個反應

Superoxide anion scavenging rate D% =(∆∆ODControl - ∆∆ODSample)÷ ∆∆ODControl ×100%

貨號KTB1090

清除氫氧自由基能力測定試劑

48個反應/

96個反應

吸收光 536nm Hydroxyl radical scavenging rate D% =(ASample - AControl)÷(ABlank - AControl)×100%

貨號KTB2220

TC總膽固醇檢測試劑盒

48個反應/

96個反應

500nm Standard: 5 μmol/mL

貨號KTB1510

CheKine™尿酸(UA)比色分析試劑盒

48個反應/

96個反應

 吸收光505 nm

Standard: 5 μmol/mL.

貨號KTB1300

Glucose葡萄糖測定試劑盒

96個反應/

192個反應

 吸光值630nm

4- 300 mg/dL)

貨號KTB1100

Lactate  乳酸檢測試劑盒 

96個反應/

480個反應

colorimetric (450 nm) 

0.03 mM-2 mM

貨號KTB1700

組織和血液鹼性磷酸酶( Alkaline Phosphatase, AKP / ALP)比色測定

96個反應/

96 X5盤

吸收光510 nm

貨號KTB1500  

總抗氧化劑能力TCA分析

96個反應/

480個反應

593 nm

偵測範圍: 0.15-3 mM

貨號KTA0002

Annexin V-AbFluor™488細胞凋亡檢測試劑盒

20個反應/50個反應/100個反應

Annexin V- AbFluor™ 488: Abs/Em = 491/517 nm; PI: Abs/Em = 535/617 nm (with DNA) 

貨號KET7013

EliKine™ Rat IL-6 ELISA Kit試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍: 250pg/mL-16000 pg/mL, 最低靈敏度100 pg/mL

貨號KET0003  

雌二醇(Estradiol , E2)試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍40 pg/ml-1500 pg/ml

貨號KET6032

TNF-α Alpha型腫瘤壞死因子 ELISA試劑盒

96個反應/

96 X5盤

450 nm

7.8 pg/ml-500 pg/ml

貨號KET6033

VEGF血管內皮生長因子ELISA試劑盒 

96個反應/

96 X5盤

450 nm

偵測範圍: 31.25 pg/mL-2000 pg/mL

貨號KET7005

白介素-1β(Interleukin-1β, IL-1β)ELISA試劑盒

96個反應/

96 X5盤

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

偵測範圍: 15.6 pg/ml-1000 pg/ml

貨號KTE70003

小鼠黃嘌呤脫氫酶/氧化酶(XDH)ELISA試劑盒

96個反應/

96 X5盤

450 nm

偵測範圍: 22.5 ng/L-360 ng/L, 最低靈敏度1.0 ng/L。

貨號 A02051HRP

His-tag標籤多株抗體-HRP 

50ul/200ul

WB (1:5000).

貨號A02170MGB

Anti-V5 Tag Mouse Monoclonal Antibody (11D5), 磁珠

100ul/400ul/2ml

GKPIPNPLLGLDST epitope

貨號KTA4001

JC-1線粒體膜電位測定試劑盒

100個反應/

500個反應

(聚結的JC-1,Ex / Em = 585/590 nm)發出綠色螢光(JC-1單體,Ex / Em = 510/527 nm)

JC-1 Stain

CCCP (10 mM)  Assay Buffer (5×)

貨號KTB1600

穀胱甘肽(Glutathione)檢測

96個反應

412 nm  2-200 µg/mL

貨號BMD00063

DAPI Staining Solution

1MG/10MG/100MG

λEx/λEm: 358/461 nm (with DNA)

貨號KTP3001

細胞核/細胞質分離試劑盒

50個反應/

200個反應

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) • Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)

貨號BMD00064-50UG

Calcein AM鈣黃綠素活細胞螢光染劑

50ug/1mg/10mg

λEX/λEm: 494/517 nm (pH 8) (calcein)

 

貨號 KTB1660

硫氧還蛋白過氧化物酶(TPX)檢測 

96個反應

240nm

貨號KTB1020

NAD/NADH 分析試劑盒

96個反應/

480個反應

OD565 nm

96 孔板測定中的檢測限值為 0.78 μM 線性最高 50 μM NAD+/NADH。

貨號KTB1070

CheKine™黃嘌呤氧化酶檢測

96個反應/

480個反應

OD 450 nm

線性範圍15.6 -500 mU/mL

貨號KTB1040

Catalase過氧化氫酶活性檢測試劑盒

96個反應/

480個反應

OD 540 nm

2-75 µM

貨號KTB1060

過氧化物酶(Peroxidases)分析試劑盒

96個反應

OD 664 nm

線性範圍為15.6-1000 µU/mL

貨號KTB1050

脂質過氧化檢測試劑盒(MDA)

96個反應

OD 532 nm

1-100 µM/L

貨號KTB1030

超氧化物歧化酶(SOD)活性測定試劑盒 

96個反應

200-3.13 U/ml

貨號KTA1030

LDH細胞毒性測定試劑盒

96個反應/

480個反應

 OD 565 nm

10,00-100,0000 cells per well

貨號KTB3010

CheKine™ 花青素還原酶(ANR)活性比色測定試劑盒

48個反應/

96個反應

absorption at 340 nm

貨號KTB1542

Tannase(TAN) 活性比色測定試劑盒 

48個反應/

96個反應

absorbance at 270 nm

0.3125-20 μmoL/mL

貨號KTB1541

CheKine™ 單寧比色測定試劑盒 | CheKine™ Tannin Colorimetric Assay Kit

48個反應/

96個反應

absorbance at 760 nm

0.0156-1 mg/mL

貨號KTB2250

CheKine™ 微量高密度脂蛋白膽固醇 (HDL-C) 檢測試劑盒

48個反應/

96個反應

Absorption peak at 500nm

Detection range: 0.078-5 mmol/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol/L)

貨號KTB2260

CheKine™ 微量低密度脂蛋白膽固醇 (LDL-C) 檢測試劑盒

48個反應/

96個反應

Absorption peak at 500nm

Detection range: 0.078-5 mmol/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol/L)

貨號KTB1290

CheKine™ 烏頭酸酶 (ACO) 活性比色測定試劑盒

48個反應/

96個反應

absorption at 340 nm.

貨號KTB1560

醇醯基轉移酶(AAT)活性比色檢測試劑盒 

48個反應/

96個反應

absorption at 412 nm.

貨號KTB1390

CheKine™ 澱粉分支酶 (SBE) 活性比色測定試劑盒

48個反應/

96個反應

absorption at 660 nm

貨號PRP1012

Human IL-6 protein重組蛋白 

5ug/20ug/100ug/1mg

human IL-6 (NP_000591.1) (Val 30-Met 212)/ E. coli

貨號PRP100273

Human IL-4 protein重組蛋白

20ug/100ug/1mg

ED50 for this effect is 0.05-0.2 ng/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 × 10E4 IU/μg.

貨號PRP100159

Human EGF protein重組蛋白

100ug/1mg

 

貨號KTB1041

CheKine™ 過氧化氫 (H2O2) 檢測試劑盒

96個反應/

480個反應

OD 580 nm

: 1 µM-100 µM

貨號KTB1210

CheKine™ 超氧陰離子比色測定試劑盒

48個反應/

96個反應

absorption peak at 540nm

0.0125-0.5 mg/mL

 

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