IgY Eggs Press
Purification Kit

IgY抗體純化---產品描述

卵黃抗體EggsPress純化套組。

1. 快速、有效地純化IgY抗體（IgY），不喪失活性。

2. 其不受卵黃脂質的影響，有效純化IgY抗體。

3. 步驟簡單。二個步驟即可純化IgY抗體。

4. 純度高。純化90％純度IgY片段。

5. 產量高。每ml的IgY蛋黃可提供4和7毫克的IgY含量。

polyethylene
glycol (PEG) – free。避免PEG移除之問題。及抗體分析之干擾

· Kit
Description

· Why Choose
our IgY Purification Kit?

· Kit
Contents

· Kit Storage
Conditions

· What You
Will Need

· Separate
Kit Components Available

· Kit and Kit
Components Costs

· Kit
Instructions

· Immunization
Protocol for Chickens

· IgY Purification
Record

產品規格

5 egg yolk isolations (Cat. No. IK 500)

· Reagent
A (500 ml)

· Reagent
B (500 ml)

· Egg
Separator

· Instructions

20 egg yolk isolations (Cat. No. IK 2000)

· Reagent
A (4 x 500 ml)

· Reagent
B (4 x 500 ml)

· Egg
Separator

· Instructions

80 egg yolk isolations (Cat. No. IK 8000)

· Reagent
A (2 x 3.8 Litres)

· Reagent
B (2 x 3.8 Litres)

· Egg
Separator

· Instructions

Kit Storage Conditions

· Keep at
room temperature until ready to use.

· Reagents
A and B must be refrigerated before use.

What you will need

· Egg
yolks

· Distilled
Water

· Phosphate-buffered
saline (PBS)

· Pasteur
pipette

· Balance

· Beaker,
graduated cylinder

· Stir
plate and stir bar

· Refrigerator

· Centrifuge

Separate Kit Components Available

Perhaps a 90% pure IgY preparation is not necessary for your experimental
procedure. Delipidation of the egg yolk provides the researcher with a
diluted IgY solution (also containing other yolk proteins) which,
concentrated and dialyzed, can be used for many applications. To delipidate
your egg yolk only, egg separators and Reagent A are available separately
(see below).

Reagent B, our IgY precipitation reagent, is also available separately to
purify IgY from other yolk proteins after delipidation.

Kit and Kit Components Costs

Product	Code
EggsPress IgY Purification Kit (5 yolks)	IK 500
EggsPress IgY Purification Kit (20 yolks)	IK 2000
EggsPress IgY Purification Kit (40 yolks)	IK 4000
EggsPress IgY Purification Kit (80 yolks)	IK 8000
Reagent A (Delipidation) (5 yolks)	RA500
Reagent A (Delipidation) (20 yolks)	RA2000
Reagent A (Delipidation) (40 yolks)	RA4000
Reagent A (Delipidation) (80 yolks)	RA8000
Reagent B (IgY Precipitation) (5 yolks)	RB500
Reagent B (IgY Precipitation) (20 yolks)	RB2000
Reagent B (IgY Precipitation) (40 yolks)	RB4000
Reagent B (IgY Precipitation) (80 yolks)	RB8000
Egg Separator	ES001

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【連絡信箱】taiding.biotech@msa.hinet.net

【台北公司】02-86609496 【台中公司】0935-229803 陳俊豪

【公司網址】
www.biopioneer.com.tw

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Anti-Digoxigenin
DIG地高辛抗體, HRP標誌

Code: ADG-HRP

非放射線標定技術製備探針。常將labeled
nucleic acid點在membrane上，並使用digoxigenin（DIG）、DNP or
Biotin antibody進行偵測。

Host:

Chicken

Volume:

0.2 ml

Quantity:

0.1 mg

Type:

HRP conjugated

Full Name:

HRP-conjugated Affinity
Purified Chicken anti-Digoxigenin

Physical State:

liquid

Concentration:

0.5 mg/ml

Diluent:

Hepes-Saline with 0.1%
BSA, 50% Glycerol

Preservative:

0.005% Thimerosal

Procedure:

Chickens were immunized
with Digoxigenin coupled to KLH. After multiple immunizations in Freund's
adjuvant, eggs were collected and the immunoglobulin fraction prepared.
Antibodies were immunoaffinity purified using the Digoxigenin immobilized on
a solid support and conjugated to horseradish peroxidase (HRP).

Specifications:

This antibody is suitable
for ELISA and Western blots. Recommended dilution for Western blots is 1:500.
For ELISA, recommended dilution is 1:5,000.

Storage:

4°C or -20°C for long-term
storage. Don't freeze-thaw often.

Expiration Date:

0000-00-00

Note:

For research use only.

Anti-Biotin抗體, HRP/AP標誌

http://tw.myblog.yahoo.com/taiding2000/article?mid=659&prev=661&next=658&l=f&fid=19

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【連絡信箱】taiding.biotech@msa.hinet.net

【台北公司】02-86609496 【台中公司】0935-229803 陳俊豪

【公司網址】
www.biopioneer.com.tw

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單株抗體生產中，融合瘤的篩選和鑒別為最重要的步驟，Southerbiotech提供SBA Clonotyping system全套組。簡單、快速、方便的ELISA檢測方法。

小鼠---單株抗體---分型ELISA套組

目錄號	產品說明	規格
5300-01	Mouse Immunoglobulin Panel	1 kit
5300-04	SBA Clonotyping System/AP	1 kit
5300-05	SBA Clonotyping System/HRP	1 kit

小鼠Ig同型對照Panel
(5300-01)

包括小鼠IgM,IgG1, IgG2a, IgG2b, IgG3各 0.1mg

SBA克隆分型系統/AP (5300-04)

以AP標記抗體，PNPP為酶底物

SBA

克隆分型系統/HRP (5300-05)

以HRP標記抗體，ABTS為酶底物

根據需求選擇5300-01和5300-04，5300-05中任何一種

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382 taiding.biotech@msa.hinet.net

【臺北公司】02-86609496 【台中公司】0935-229803

【相關網址】 www.biopioneer.com.tw

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---------終點顯色法(Chromogenic LAL Endotoxin Assay)產品介紹----

內毒素檢測試劑(LAL Reagent) Gel-Colt(凝膠法) 鱟試劑與內毒素反應會產生凝膠，利用凝膠的生成與否來偵測內毒素的方法，為最早發展出來的LAL測試方法。

endotoxin_assay_kit

Genesript提供ToxinSensor 凝膠法 (Gel clot Endotoxin Assay kit)和終點顯色法(Chromogenic LAL Endotoxin Assay)，兩種內毒素檢測試劑盒，可以對人或動物的注射用藥、生物製品及醫療設備的終端產品中的內毒素進行檢測。

內毒素檢測試劑

Genescript 提供二種型式，之內毒素檢測試劑。

ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (終點顯色法)

訂購貨號: Cat. No. L00350

產品名稱: ToxinSensor™ Chromogenic LAL
Endotoxin Assay Kit

靈敏度: Max Sensitivity: 0.005 EU/ml, or less

使用儀器: color spectrophotometer;

ToxinSensor™ Gel clot Endotoxin Assay Kit (凝膠法)

訂購貨號: Cat. No. L00351

產品名稱: ToxinSensor™ Gel clot Endotoxin
Assay Kit

靈敏度: 0.25 EU/ml

使用儀器: 37°C water bath

ToxinSensorTM終點顯色法內毒素檢測試劑盒

一種快速高敏感的終點顯色LAL檢測

ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (終點顯色法)

使用儀器: color spectrophotometer;

優點

ToxinSensorTM終點顯色法內毒素檢測試劑盒的作用原理是在LAL測試中用內毒素催化酶原的活性。這樣可以分離出無色物質並產生有色的最終產物，該產物可以在分光光度計中545 nm光照處被檢測到，並與標準物質曲線進行對照。

高重複性和高線性相關

我們分別用X公司和genescript公司終點顯色法內毒素檢測試劑盒對濃度範圍為0.1-1 EU/ml的內毒素標準品進行檢測，結

果如下：

產品規格

ToxinSensorTM 終點顯色法內毒素檢測試劑盒1 kit (16次) L00350C

ToxinSensorTM 終點顯色法內毒素檢測試劑盒1 kit (32次) L00350

ToxinSensor^TM Gel Clot Endotoxin Assay Kit: It is a qualitative test for Gram-negative bacterial endotoxin. Limulus Amebocyte Lysate as supplied is to be reconstituted with LAL Reagent Water and then mixed in equal parts with the solution being tested. After incubation, and in the presence of endotoxin, gelation occurs; in the absence of endotoxin, gelation does not occur.

Click to load FAQs

Ordering Information

Cat. No.

Product Name

Size

Price

Quantity

ToxinSensor™ Single Test Kit: is designed to be a qualitative In Vitro end-point endotoxin test for human and animal parenteral drugs, biological products, and medical devices.

Limulus Amebocyte Lysate (LAL) as supplied is to be reconstituted with sample or control directly. There is no need to reconstitute the system with LAL Reagent Water before use. After incubation for 1 hour, and in the presence of endotoxin, gelation occurs; in the absence of endotoxin, gelation does not occur.

GenScript supply a series of ToxinSensor™ Single Test Kits with different sensitivity (0.015 EU/ml, 0.03 EU/ml, 0.06 EU/ml, 0.125 EU/ml and 0.25 EU/ml).

When using the ToxinSensor™ Single Test Kit, you just need to add 200µl of sample or control to LAL, then, wait for the required incubation time to allow for gel to be formed.

Cat. No.

Product Name

Size

Price

Quantity

Assay Example :

Each assay should include both a positive control and a negative control (LAL Reagent Water). Following are application example:

Sample: Protein A, 1 mg/ml in PBS, pH 7.4. It is purified from E. coli sonicate by Ni-NTA resin.
Dilution by LAL water: 1: 200,000, 1: 400,000, 1: 800,000
The test is performed as described above and the assay result is:

Positive control Negative control 1: 200,000 1: 400,000 1: 800,000

+

-

+

Endotoxin concentration in this sample is: from 200,000×0.25 to 400,000×0.25, that is, from 50,000 to 100,000 EU/ml.

相關內毒素檢測試劑

---------凝膠法 (Gel clot Endotoxin Assay kit)產品---

http://tw.myblog.yahoo.com/taiding2000/article?mid=847&prev=-1&next=846

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【臺北公司】02-86609496 【台中公司】0935-229803

【相關網址】 www.biopioneer.com.tw

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Oct 31 Wed 2012 23:16
【內毒素檢測試劑LAL Reagent】Genescript 貨號#L00351。 ToxinSensor 凝膠法 (Gel clot Endotoxin Assay kit)

---------凝膠法 (Gel clot Endotoxin Assay kit)產品介紹----

內毒素檢測試劑(LAL Reagent) Gel-Colt(凝膠法) 鱟試劑與內毒素反應會產生凝膠，利用凝膠的生成與否來偵測內毒素的方法，為最早發展出來的LAL測試方法。

endotoxin_assay_kit

Genesript提供ToxinSensor 凝膠法 (Gel clot Endotoxin Assay kit)和終點顯色法(Chromogenic LAL Endotoxin Assay)，兩種內毒素檢測試劑盒，可以對人或動物的注射用藥、生物製品及醫療設備的終端產品中的內毒素進行檢測。

內毒素檢測試劑---

Genescript 提供二種型式，之內毒素檢測試劑。

ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (終點顯色法)

訂購貨號: Cat. No. L00350

產品名稱: ToxinSensor™ Chromogenic LAL
Endotoxin Assay Kit

靈敏度: Max Sensitivity: 0.005 EU/ml, or less

使用儀器: color spectrophotometer;

ToxinSensor™ Gel clot Endotoxin Assay Kit (凝膠法)

訂購貨號: Cat. No. L00351

產品名稱: ToxinSensor™ Gel clot Endotoxin
Assay Kit

靈敏度: 0.25 EU/ml

使用儀器: 37°C water bath

ToxinSensorTM凝膠法內毒素檢測試劑盒

ToxinSensor™ Gel clot Endotoxin Assay Kit (凝膠法)

訂購貨號: Cat. No. L00351

使用儀器: 37°C water bath

優點

1小時內完成所有過程

高重複性

靈敏度為0.25 EU/ml

試劑隨時可用，並附有不含內毒素的槍頭、試管

簡單省時的過程

每一個測試應該包括一個陽性對照和一個陰性對照 (LAL試劑)。應用實例如下：

1. 樣品：1 mg/ml的蛋白質A，被置於pH 7.4的磷酸緩衝液中。蛋白質A是由Ni-NTA樹脂純化大腸桿菌得到。

2. 按照稀釋比例1: 200,000，1: 400,000，1: 800,000在LAL試劑水中稀釋。

3.該測試是在上述條件下進行，得到的結果如下：

4.初步判斷該樣品的內毒素在50,000到100,000 EU/ml之間

產品規格

ToxinSensorTM 凝膠法內毒素檢測試劑盒1 kit (40次)L00351

Endotoxin Assay Kits

Endotoxin test is the most critical quality control test required by the FDA for all drugs in their final stages of formulation. Endotoxins are invariably associated with every gram-negative bacteria, so they cause severe reactions in humans and animals and retain high toxic activity even present at low concentration. In addition, endotoxins are suspected to play an important role in the occurrence and development of many different diseases.

GenScript strongly recommends innovative detection technique to detect endotoxin. GenScript's Endotoxin Assay Kits can be well used in in vitro end-product endotoxin test for human and animal parenteral drugs, biological products, and medical devices.

Key Features

Good linearity and good reproducibility
Ready-to-use reagents and materials, such as tips, endotoxin-free tubes,etc.
High sensitivity and broad application range
Competitive price

ToxinSensor^TM Chromogenic LAL Endotoxin Assay Kit: The kit utilizes a modified Limulus Amebocyte Lysate and a synthetic color producing substrate to quantitatively detect endotoxin chromogenically in a broad range (0.005–1 EU/ml).

Note:GenScript has launched a new size (32 rxns) with reasonable price on 03/08/2010.Related components have also been updated!

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Cat. No.

Product Name

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Price

Quantity

Technical Advantages:

Under the standard conditions, the absorbance at 545 nm shows a linear relationship with the concentration in range of both 0.01 - 0.1 EU/ml and 0.1 - 1 EU/ml. Plot the absorbance for the four standards on the x-axis, the corresponding endotoxin concentration in EU/ml on the y-axis. Draw a best-fit straight line among these points and determine endotoxin concentrations of samples graphically.

Examples using following data:

Vial No.	Sample	Absorbance at 545 nm	Δ Absorbance
1	LAL reagent water (Blank)	0.035	-
2	0.01 EU/ml Standard	0.088	0.053
3	0.025 EU/ml Standard	0.133	0.098
4	0.05 EU/ml Standard	0.217	0.182
5	0.1 EU/ml Standard	0.427	0.392

If the mean absorbance of a sample is x, the endotoxin concentration of sample will be 0.2618x – 0.0012 EU/ml. All incubations were performed for 45 min. Above is only an example curve show, and the OD values of standards may be different with different assays.

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【臺北公司】02-86609496 【台中公司】0935-229803

【相關網址】 www.biopioneer.com.tw

相關內毒素檢測試劑

---------終點顯色法(Chromogenic LAL Endotoxin Assay)介紹----

http://tw.myblog.yahoo.com/taiding2000/article?mid=848&prev=-1&next=847

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Oct 31 Wed 2012 12:29
【酵素分解蛋白】Enzyme - Product Navigation for Enzyme Related Topics。SINO BIOLOGICAL

Enzyme - Product Navigation for Enzyme Related Topics

資料連結如下:

http://www.sinobiological.com/Enzyme-a-1132.html

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Oct 29 Mon 2012 21:17
【Biotin標誌之E.coli 大腸桿菌抗體】貨號#Y051802---Biotin標誌--- Anti-Escherichia Coli (E- Coli) Antibody。Abmgood

Anti-Escherichia Coli (E- Coli)
Antibody, biotin label

大腸桿菌抗體, 100ul

---Biotin標誌---

Cat.No.:	Y051802
Quantity:	100μl
Antibody Type	Primary Antibody
Description	Anti-Escherichia coli Biotin Conjugate is a This product consists of purified IgG fraction of the above antiserum covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.Protein concentration is 4-5 mg/ml.
Immunogen	Mixtures of all antigenic serotypes
Species Reactivity	CROSS REACTIVITY: Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae UNINFECTED CELL REACTIVITY: Not applicable Will remove E. coli proteins from recombinant preparations OTHER
Specificity	All "O" and "K" antigenic serotypes of Escherichia coli
Tested Applications	TITER : > 1:1,000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Application Notes	added. Possible applications for this product include avidin and streptavidin amplification systems for immunohistochemistry, ELISA, fluorescence microscopy and immunoblotting. In addition, this product may be used in place of neat antiserum in almost any appropriate antibody-based technique.
Raised In	Rabbit
Clonality	Polyclonal
Isotype	IgG
Purity	purified IgG fraction
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Formulation	Liquid
Concentration	1ug/ul
Storage	Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
Caution	Anti-Escherichia coli Biotin Conjugate is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

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Oct 29 Mon 2012 21:03
【相關E.coli 大腸桿菌抗體】abmgood anti-Escherichia coli antiobody

相關大腸桿菌抗體

Product	Size	Concentration	Cat. No.
Anti-Escherichia coli Biotin Conjugate	100μl	1ug/ul	Y051802
Anti-Escherichia coli Fluorescein Conjugate	100μl	1ug/ul	Y051803
Anti-Escherichia coli Peroxidase Conjugate	100μl	1ug/ul	Y051804
Anti-Escherichia coli Unconjugated Preparation Goat	100μl	1ug/ul	Y051805
Anti-Escherichia coli Biotin Conjugate Goat	100μl	1ug/ul	Y051806
Anti-Escherichia coli Fluorescein Conjugate Goat	100μl	1ug/ul	Y051807
Anti-Escherichia coli Peroxidase Conjugate Goat	100μl	1ug/ul	Y051808
Anti-Escherichia coli O26:B6:H11	100 μl	1ug/ul	Y052573
Anti-Escherichia coli O26:B6	100 μl	1ug/ul	Y052574
Escherichia Coli (E. Coli)	100 ug		Y102286
Escherichia Coli (E. Coli) O157	100 ug		Y102314
Escherichia Coli (E. Coli) O157	100 ug		Y102315
Escherichia coli Shigatoxin (E. coli)	1 mg		Y102537
Escherichia coli Shigatoxin (E. coli)	1 mg		Y102538
Escherichia coli Shigatoxin (E. coli)	1 mg		Y102539
Escherichia coli (E. coli)	1 mg		Y102540
Escherichia coli (E. coli)	1 mg		Y102541
Escherichia coli (E. coli) O157	100ug		Y102542
Escherichia Coli (E. Coli) 0157:H7	100ug		Y102543
Escherichia coli (E. coli) K99 Pilus	1 mg		Y102548
Escherichia coli, Shigatoxin (E. coli)	1mg		Y104138
Escherichia Coli Shigatoxin (E. Coli)	1mg		Y104139
Escherichia Coli Shigatoxin (E. Coli)	1mg		Y104140
Escherichia coli (E. coli)	1mg		Y104141
Escherichia coli (E. coli)	1mg		Y104142
Escherichia coli (E. coli) O157	100ug		Y104143
Escherichia coli (E. coli) 0157:H7	100ug

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個人分類：【重組蛋白、單多株抗體製備純化】

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Oct 29 Mon 2012 20:47
【E.coli 大腸桿菌抗體】貨號#Y051805。Anti-Escherichia Coli (E- Coli)。abmgood

Anti-Escherichia Coli (E- Coli)
Antibody

大腸桿菌抗體, 100ul

Cat.No.:	Y051805
Quantity:	100μl

NT$11,000元

Antibody Type	Primary Antibody
Description	Anti-Escherichia coli Unconjugated Preparation Goat is a This product consists of the purified IgG fraction of the above antiserum prepared by methods to a purity of >95%.It is also suitable for conjugation purposes. Protein concentration is 4-5 mg/ml.
Immunogen	Mixture of E. coli serotypes
Species Reactivity	CROSS REACTIVITY: Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae UNINFECTED CELL REACTIVITY: Not applicable
Specificity	Many "O" and "K" antigenic serotypes of Escherichia coli
Tested Applications	TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Application Notes	Potential applications for this product are numerous including ELISA, fluorescence microscopy, immunoblotting and immunohistochemistry. In addition, this product may be used in place of neat antiserum in almost any appropriate antibody-based technique.
Raised In	Goat
Clonality	Polyclonal
Isotype	IgG
Purity	purified IgG fraction
Storage Buffer	PBS, pH 7.4 with 0.05% sodium azide.
Formulation	Liquid
Concentration	1ug/ul
Storage	Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
Caution	Anti-Escherichia coli Unconjugated Preparation Goat is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

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Oct 19 Fri 2012 00:14
【AbSelec TCS融合瘤組織培養液之純化】 Mouse TCS抗體純化(832-0500)。Rabbit/human TCS抗體純化 (862-0500)

抗體純化套組---TCS融合瘤組織培養液

AbSelect Mouse
TCS Purification System (832-0500)

AbSelect TCS
Antibody Purification System (862-0500)

融合瘤組織培養液(TCS)產製抗體之純化

AbSelect TCS Antibody Purification System (832-0500)/(862-0500)

Innova Bioseiences提供一系統抗體純化套組，每組套組包括抗體純化之buffer及resin，有效純化不同來源之抗體含量。其純化來源，可為市售抗體之純化(貨號#860-0010/rabbit; 貨號#830-0010/mouse; 貨號#890-0010/rat/sheep/human/goat)、腹水及血清產製抗體之純化(貨號#863-0500/rabbit; 貨號#893-0500/rat/sheep/human/goat/mouse)、融合瘤組織培養液(TCS)產製之抗體(貨號#862-0500/rabbit/human; 貨號#832-0500/mouse)、低濃度抗體之純化及濃縮(貨號#861-0010)。有效移除溶液中BSA、Glycine、Tris、Azide等殘留分子，純化後抗體溶液，可直接應用於lateral flow test奈米金抗體標誌套組 (Innova gold,#230-0010, innova biosciences)、與HRP/螢光抗體標誌套組(Innova lightning-link, #701-0000)。

------奈米金抗體標誌 (InnovaCoat)----

------HRP/螢光抗體標誌 (lightning-link)----

腹水、血清純化抗體à AbSelect TCS組織培養液抗體------純化系統

適合應用: 融合瘤組織培養液(TCS)產製之抗體。

純化來單株細胞產製之抗體，移除培養液中殘留之amino group。純化後之抗體，可直接進行奈米金抗體標誌套組 (Innova gold,#230-0010, innova biosciences)及抗體螢光或HRP標誌(lightning_link)。

AbSelect TCS Antibody Purification System

Antibodies are often generated from hybridoma cell lines
and supplied in tissue culture supernatant (TCS). TCS itself contains many substances
that interfere with antibody labeling reactions. Successful labeling therefore
requires antibodies to be purified prior to labeling. The AbSelect TCS system
is a fast and simple method to purify these antibodies.

AbSelect TCS is prepared by coupling highly purified
protein A to agarose beads which can be used to purify IgG fractions from
hybridoma supernatants. The method involves the capture of the antibody on the
AbSelect TCS resin and the removal of unwanted substances using a simple wash
procedure. The purified product is then eluted and neutralized.

The components of the AbSelect TCS are fully compatible
with the Lightning-Link™ antibody labeling kits (available separately),
which allows the purified antibody to be immediately labeled with an enzyme,
fluorescent protein or fluorescent dye, with a hands-on time of under 30
seconds.

【email信件】 taiding.biotech@msa.hinet.net

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【台北公司】02-86609496 【台中公司】0935229803

【相關網址】www.biopioneer.com.tw

【原廠網址】http://www.innovabiosciences.com

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抗體純化套組---腹水及血清產製抗體

AbSelect Serum Antibody Purification System (863-0500)

AbSelect G Serum Antibody Purification System (893-0500)

AbSelect Serum Antibody
Purification System (863-0500)/(893-0500)

Innova Bioseiences提供一系統抗體純化套組，每組套組包括抗體純化之buffer及resin，有效純化不同來源之抗體含量。其純化來源，可為市售抗體之純化(貨號#860-0010/rabbit; 貨號#830-0010/mouse; 貨號#890-0010/rat/sheep/human/goat)、腹水及血清產製抗體之純化(貨號#863-0500/rabbit; 貨號#893-0500/rat/sheep/human/goat/mouse)、融合瘤組織培養液(TCS)產製之抗體(貨號#862-0500/rabbit/human; 貨號#832-0500/mouse)、低濃度抗體之純化及濃縮(貨號#861-0010)。有效移除溶液中BSA、Glycine、Tris、Azide等殘留分子，純化後抗體溶液，可直接應用於lateral flow test奈米金抗體標誌套組 (Innova gold,#230-0010, innova biosciences)、與HRP/螢光抗體標誌套組(Innova lightning-link, #701-0000)。

------奈米金抗體標誌 (InnovaCoat)----

------HRP/螢光抗體標誌 (lightning-link)----

腹水、血清純化抗體AbSelect 血清抗體------純化系統

適合應用: 腹水及血清產製之抗體。

純化來自培養液、腹水及血清之抗體，移除殘留之amino group。純化後之抗體，可直接進行奈米金抗體標誌套組 (Innova gold,#230-0010, innova biosciences)及抗體螢光或HRP標誌(lightning_link)。

AbSelect Serum Antibody Purification System

Antibodies generated from ascites fluid and serum are
often supplied as crude formulations. Both ascites fluid and serum contain many
substances that interfere with antibody labeling reactions. Successful labeling
therefore requires antibodies to be purified prior to labeling. The AbSelect
Serum system is a fast and simple method to purify antibodies from these types
of media.

AbSelect Serum is prepared by coupling highly purified
protein A to agarose beads which can be used to purify IgG fractions from
ascites fluid and serum. The method involves the capture of the antibody on the
AbSelect Serum resin and the removal of unwanted substances using a simple wash
procedure. The purified product is then eluted and neutralized.

The components of the AbSelect Serum purification system
are fully compatible with the Lightning-Link™ antibody labeling kits (available
separately), which allows the purified antibody to be immediately labeled with
an enzyme, fluorescent protein or fluorescent dye, with a hands-on time of
under 30 seconds.

【email信件】 taiding.biotech@msa.hinet.net

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【台北公司】02-86609496 【台中公司】0935229803

【相關網址】www.biopioneer.com.tw

【原廠網址】http://www.innovabiosciences.com

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抗體純化套組---市售抗體之純化

AbSelect Antibody Purification System (860-0010)

市售抗體之純化。有效移除溶液中BSA、Glycine、Tris、Azide等殘留分子

AbSelect Antibody Purification
System (860-0010)

Innova Bioseiences提供一系統抗體純化套組，每組套組包括抗體純化之buffer及resin，有效純化不同來源之抗體含量。其純化來源，可為市售抗體之純化(貨號#860-0010/rabbit; 貨號#830-0010/mouse; 貨號#890-0010/rat/sheep/human/goat)、腹水及血清產製抗體之純化(貨號#863-0500/rabbit; 貨號#893-0500/rat/sheep/human/goat/mouse)、融合瘤組織培養液(TCS)產製之抗體(貨號#862-0500/rabbit/human; 貨號#832-0500/mouse)、低濃度抗體之純化及濃縮(貨號#861-0010)。有效移除溶液中BSA、Glycine、Tris、Azide等殘留分子，純化後抗體溶液，可直接應用於lateral flow test奈米金抗體標誌套組 (Innova gold,#230-0010, innova biosciences)、與HRP/螢光抗體標誌套組(Innova lightning-link, #701-0000)。

------奈米金抗體標誌 (InnovaCoat)----

------HRP/螢光抗體標誌 (lightning-link)----

BSA、Gelatin、Glycine移除--AbSelect 抗體-----純化系統

適合應用: 市售抗體。

預移除溶液中BSA、Glycine、Tris、Azide等殘留分子

純化體積100ul至500ul

AbSelect抗體純化套組 (860-0010), 特別針對市售抗體保存液內, 含有BSA, Glycine. Tris. Azide等amino group干擾物質殘留。純化後的抗體, 可直接應用於奈米金抗體標誌套組 (Innova gold,#230-0010)及抗體標誌試劑 (lightning-link一個步驟完成抗體標誌試驗(lightning-link,
#701-0000)。

不須經由dialysis等純化步驟。抗體的體積可經由100ul至0.5ml，每次純化, 可以純化>500ug抗體量。

Ps. 蛋白質濃縮及buffer exchange之動作, 無法有效移除抗體內的小分子, 須經由Abselect
抗體純化系統來執行。

AbSelect訂購貨號

1個純化套組, 貨號#860-0005

3個純化套組, 貨號#860-0010

AbSelect套組內容物

Lyophilised AbSelect Protein A resin

1 vial of 10x Binding Buffer

1 vial of Wash Buffer

1 vial of Elution Buffer

1 vial of Neutralization Buffer

1 or 3 spin cartridge/collecting tube assemblies

4 or 12 additional collecting tubes

AbSelect抗體純化流程

Step 1 取Wash buffer重組Protein A resin

Step 2 將重組好的Resin, 移至離心管, 並spin down

Step 3 抗體加入10x Binding Buffer後,移入管柱中反應1個小時

Step 4 Wash resin.

Step 5 Elute 及neutralize約化的antibody.

Step 6 確認elute的抗體含量, 並以蛋白質定量.

AbSelect Antibody Purification System

市售抗體之純化(貨號#860-0010)

Commercially available antibodies often
contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling
reactions. The AbSelect™ Purification Kit quickly removes these contaminants.
It can also be used to purify antibodies from crude samples such as ascites
fluid or immune serum.

The antibody to be purified or cleaned up ideally is in a
volume of 100ul to 0.5ml. Up to 500µg of antibody can be purified in each run.
The AbSelect method involves capture of the antibody on protein A resin and the
removal of unwanted substances by a simple wash procedure. The purified product
is then eluted and neutralized.

The components of the AbSelect kit are fully compatible
with the Lightning-Link™
antibody labeling system (available separately), which allows the purified
antibody to be rapidly conjugated with a wide variety of labels.

【email信件】 taiding.biotech@msa.hinet.net

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【台北公司】02-86609496 【台中公司】0935229803

【相關網址】www.biopioneer.com.tw

【原廠網址】http://www.innovabiosciences.com

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Oct 04 Thu 2012 10:24
【老鼠Galectin-1(Gal1)重組蛋白,】Recombinant Protein 貨號# 50100-MNAE，Galectin-1/GAL1/LGALS1，with Native

*** --老鼠Galectin-1(Gal1)重組蛋白, 50100-MNAE****

Mouse Galectin-1 / 14 kDa laminin-binding
protein Protein

Mouse Lgals1 /
Galectin-1 Protein Price Inquiry ( Available Sizes )

50100-MNAE

訂貨資料50100-MNAE

·
500μg: Inquiring Price;

·
≥1mg Bulk:

Inquiring Price

Lgals1 / Galectin-1 Protein Product
Information

Synonym :	AA410090, Gal-1, Galbp, L-14.5, L14, Lect14, galectin-1
Protein Construction:	A DNA sequence encoding the mouse Lgals1 ( P16045 ) ( Met 1 - Glu 135 ) was expressed and purified
Source:	Mouse
Expression Host:	E.coli

產品資料------

Lgals1 / Galectin-1 Protein QC Testing

Purity:	> 95 % as determined by SDS-PAGE	SDS-PAGE: SDS-PAGE: Lgals1 protein
Bio-activity:	Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 1-5 μg/ml.
Endotoxin:	Please contact us for more information.
Stability:	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal:	Met 1
Molecular Mass:	The recombinant mouse Lgals1 consists of 135 amino acids and migrates as an approximately 15 kDa band as predicted in SDS-PAGE under reducing conditions
Formulation:	Lyophilized from sterile PBS, 100 mM β-Lactose, pH 7.4 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Lgals1 / Galectin-1 Protein Usage
Guide

Storage:	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Lgals1 /
Galectin-1 Protein Related Products & Topics

Cancer>>Angiogenesis>>Adhesion
Molecules in Angiogenesis>>Galectin-1

Immunology>>Adhesion
Molecule>>Lectin>> Galectin-1

更多Galectin 重組蛋白資料

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
Galectin-1/GAL1/LGALS1	Human	Galectin-1 Protein, Recombinant, with Native	10290-HNAE
Galectin-1/GAL1/LGALS1	Human	Galectin-1 / GAL1 Protein (Low Endotoxin), Recombinant, with Native	10290-HNAE-E
Galectin-1/GAL1/LGALS1	Mouse	Galectin-1 Protein, Recombinant, with Native	50100-MNAE
Galectin-1/GAL1/LGALS1	Rat	LGALS1 / Galectin-1 Protein, Recombinant, with Native	80248-RNAE

Antibodies:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
Galectin-1/GAL1/LGALS1	Human	Galectin-1 Antibody (Antigen Affinity Purified)	10290-RP02

Lgals1 /
Galectin-1 Protein Description

Mouse Galectin-1, also known
as 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding
lectin L-14-I, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1,
S-Lac lectin 1 and LGALS1, is a secreted protein which contains one galectin
domain. Galectins are a family of structurally related carbohydrate-binding
proteins, which are defined by their affinity for
poly-N-acetyllactosamine-enriched glycoconjugates and sequence similarities in
the carbohydrate recognition domain. Galectin-1 / LGALS1 is expressed in
placenta, maternal decidua and fetal membranes. Within placenta, it is
expressed in trophoblasts, stromal cells, villous endothelium,
syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes,
Galectin-1 / LGALS1 is expressed in amnion, chorioamniotic mesenchyma and
chorion (at protein level). Galectin-1 / LGALS1 is also expressed in cardiac,
smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. Galectin-1
/ LGALS1 may regulate apoptosis, cell proliferation and cell differentiation.
It binds beta-galactoside and a wide array of complex carbohydrates. Galectin-1
/ LGALS1 inhibits CD45 protein phosphatase activity and therefore the
dephosphorylation of Lyn kinase. Galectin-1 / LGALS1 has demonstrated a diverse
range of activities in relation to cell survival and proliferation. In
different circumstances, it acts as a mitogen, as an inhibitor of cell
proliferation, and as a promoter of cellular apoptosis.

References

1. Demydenko,D. et al., 2009,
Exp Oncol. 31 (2):74-9.

2. McGraw,J. et al., 2004, Eur
J Neurosci. 20 (11):2872-80.

3. Di Lella S., et al., 2009,
Biochemistry 48:786-791.

4. Rabinovich,G.A. 2005,Br J
Cancer. 92 (7):1188-92.

5. Scott,K. et al., 2004,
Glycoconj J. 19 (7-9):467-77.

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜 0920-312382

【台北公司】02-86609496

【相關網址】 www.biopioneer.com.tw

【原廠網址】http://www.sinobiological.com

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Oct 02 Tue 2012 16:07
【Galectin-3重組蛋白】 Prospec 重組蛋白。貨號CYT-606 LGALS1、LGALS2、LGALS3、LGALS4、LGALS7、LGALS8、LGALS9

* --Galectin-3 重組蛋白---貨號CYT-606

Galectin-3，為分子量31 kDa的蛋白質，是屬於beta-galectose-binding lectin家族的一員，能正向與負向地調節T細胞和巨噬細胞的免疫功能。Galectin-3主要分布於細胞質內，然而近期研究也在細胞核、細胞表面以及細胞外間質偵測到Galectin-3的表現。

相同字義: Galectin-3, GAL3, MAC2, CBP35, GALB, GALIG, LGALS2, LGALS3,
Galactose-specific lectin 3, Mac-2 antigen, IgE-binding protein, 35 kDa lectin,
Carbohydrate-binding protein 35, CBP 35, Laminin-binding protein, Lectin L-29,
L-31, Galactoside-binding protein, GALBP.

Galectin-3---(Gal3)---功能介紹

Galectin-3 mediates with the alpha-3, beta-1
integrin the stimulation by cspg4 of endothelial cells migration. Galectin-3
plays an necessary part during the acquisition of vasculogenic mimicry and
angiogenic properties associated with melanoma progression. LGALS3
overexpression is highly expressed in early stages of papillary carcinoma, and
its expression intensity declines during tumor progression. Serum levels of
LGALS3 are high in patients with thyroid malignancy but there is considerable
overlap in serum LGALS3 concentrations between those with benign and malignant
nodular thyroid disease. LGLAS3 takes part as an immune regulator to inhibit
T-cell immune responses and promote tumor growth, as a result providing a new
mechanism for tumor immune tolerance.

Galectin3---(Gal3)---重組蛋白描述

Description	LGALS3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 250 amino acids and having a molecular mass of 26 kDa. The LGALS3 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	The Galectin-3 protein was lyophilized from a concentrated (1mg/ml) containing 10mM sodium phosphate buffer, pH-7.5 and 50mM NaCl.
Solubility	It is recommended to reconstitute the lyophilized LGALS3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Galectin-3 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Galectin-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity	Greater than 97.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Amino acid sequence	ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI.
Biological Activity	Determined by its ability to chemoattract human blood monocytes. Chemotactic activity was observed at a concentration of 2µg/ml with a peak response obtained at 220,000ng/ml corresponding to a specific activity of 5IU/mg.
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Galectin(Gal)相關產品內容

ated Products

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【連絡信箱】taiding.biotech@msa.hinet.net

【臺北公司】02-86609496【台中公司】0935-229803 陳俊豪

【公司網址】 www.biopioneer.com.tw

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Oct 02 Tue 2012 15:50
【Galectin-1重組蛋白】 Prospec 重組蛋白。貨號CYT-544 LGALS1、LGALS2、LGALS3、LGALS4、LGALS7、LGALS8、LGALS9

*** --Galectin-1(Gal1)重組蛋白, CYT-544****

Galectin-1(Gal1)是可與β-galactoside
binding 的lectins 蛋白，具有很多的生物功能，像是細胞增生、移動、凋亡、發炎、轉移、免疫調節等。產品貨號# CYT-544, 分有50ug包裝及1mg包裝。

相同字義: Galectin-1, GAL1, GAL-1, Lectin galactoside-binding soluble 1,
Beta-galactoside- binding lectin L-14-I, Lactose-binding lectin 1, S-Lac lectin
1, Galaptin, 14 kDa lectin, HPL, HBL, Putative MAPK-activating protein PM12,
GBP, DKFZp686E23103.

Galectin-1(Gal1)功能介紹

The
galectins are a family of beta-galactoside-binding proteins implicated in
modulating cell-cell and cell-matrix interactions. Galectin-1 is an autocrine
negative growth factor that regulates cell proliferation. Galectin-1 regulates
cell apoptosis and cell differentiation. Galectin-1 binds CD45, CD3 and CD4
& inhibits CD45 protein phosphatase activity and therefore the
dephosphorylation of lyn kinase. Galectin-1 and its ligands are one of the
master regulators of immune responses as T-cell homeostasis and survival,
T-cell immune disorders, inflammation and allergies as well as host–pathogen
interactions. Galectin-1 expression or overexpression in tumors and/or the
tissue surrounding them must be considered as a sign of the malignant tumor
progression that is often related to the long-range dissemination of tumoral
cells (metastasis), to their dissemination into the surrounding normal tissue,
and to tumor immune-escape. Galectin-1 in its oxidized form plays a number of
important roles in the regeneration of the central nervous system after injury.
The targeted overexpression (or delivery) of Galectin-1 should be considered as
a method of choice for the treatment of some kinds of inflammation-related
diseases, neurodegenerative pathologies and muscular dystrophies. In contrast,
the targeted inhibition of Galectin-1 expression is what should be developed
for therapeutic applications against cancer progression. Galectin-1 is thus a
promising molecular target for the development of new and original therapeutic
tools. There is 88% homology between the human and mouse galectin-1.

Galectin-1(Gal1)重組蛋白描述

Description	LGALS1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 14.5 kDa. The LGALS1 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	The Galectin-1 protein was lyophilized from a concentrated (1mg/ml) containing 10mM sodium phosphate buffer, pH-7.5.
Solubility	It is recommended to reconstitute the lyophilized LGALS1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized Galectin-1 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Galectin-1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Amino acid sequence	ACGLVASNLN LKPGECLRVR GEVAPDAKSF VLNLGKDSNN LCLHFNPRFN AHGDANTIVC NSKDGGAWGT EQREAVFPFQ PGSVAEVCIT FDQANLTVKL PDGYEFKFPN RLNLEAINYM AADGDFKIKC VAFD.
Biological Activity	Human Galectin-1 is a chemoattractant for human blood monocytes. ED50 range is from 0.05–3.0 ug/ml corresponding to a specific activity of 0.3-20MIU/mg.
Protein content	UV spectroscopy at 280 nm using the absorbency value of 0.59 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
Usage	ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Galectin(Gal)相關產品內容

ated Products

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【連絡信箱】taiding.biotech@msa.hinet.net

【臺北公司】02-86609496【台中公司】0935-229803 陳俊豪

【公司網址】 www.biopioneer.com.tw

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Anti-HMGB1 Antibody

Purified Rabbit Anti Human
HMGB1 Polyclonal Antibody

特價供應19,500

免疫源為HMGB1重組蛋白. 應用於ELISA、WB。可測量於人類及Mouse物種

Cat.No.:	Y054927
Quantity:	100μl
Antibody Type	Primary Antibody
Pictures
Pictures
Immunogen	Recombinant Protein
Species Reactivity	Human, Mouse.Other species are not tested.Please decide the specificity by homology
Tested Applications	ELISA and WB
Application Notes	ELISA 1:1,000~100,000 Western blotting 1:100~1,000
Raised In	Rabbit
Clonality	Polyclonal
Isotype	IgG
Purity	Antigen Affinity Purified
Storage Buffer	PBS, pH 7.4 with 0.1% sodium azide, 50% Glycerol
Formulation	Liquid
Concentration	1ug/ul
Storage	-20oC, Avoid freeze / thaw cycles.
Caution	Purified Rabbit Anti Human HMGB1 Polyclonal is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

Anti-HMGB1 Antibody

HMGB1 Antibody

Cat.No.:	Y058201
Quantity:	100ul

特價供應4,750

Antibody Type	Primary Antibody
Accession #	NM_002128
Pictures
Immunogen	Synthetic peptide conjugated to KLH
Species Reactivity	Human,rat ,mus
Specificity	Not yet tested in other species.
Tested Applications	Peptide ELISA,WB,
Application Notes	For research use only Peptide ELISA titer: 1:160,000 Western blot analysis: 1:200-1000 Optimal dilutions/concentrations should be determined by the end use.
Raised In	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Purity	Affinity purified
Storage Buffer	PBS, pH 7.4 with 0.02% sodium azide.
Formulation	Liquid
Concentration	100ug/100ul
Storage	Store at -20°C/1 year
References	(1) Ge,H. and Roeder,R.G. The high mobility group protein HMG1 can reversibly inhibit class II gene transcription by interaction with the TATA-binding protein.J. Biol. Chem. 1994ï¼Œ269 (25), 17136-17140
Caution	HMGB1 is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

Anti-HMGB1 Antibody

HMGB1 Antibody

Cat.No.:	Y058450
Quantity:	100ul

特價供應4,750

Antibody Type

Primary Antibody

Pictures

Immunogen

Peptide

Species Reactivity

Human

Tested Applications

WB

Application Notes

Optimal dilutions to be
determined by researchers

Raised In

Mouse

Clonality

Monoclonal

Isotype

IgG

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HMGB1人類重組蛋白,Human

HMGB1 Human重組蛋白 ,訂購貨號 # PRO-581

產品特色

ProSpec重組蛋白活性高，其純度高於98％以上，並經由三種測定方式RP-HPLC、FPLC及SDS-PAGE來分析其純度，ProSpec生產之重組蛋白其內毒素小於0.1ng/ug，可進行活體實驗。ProSpec產品種類超過千種，產品涵蓋；生長激素(Cytokines), 趨化因子(Chemokines), 生長因子(Growth Fectors), 荷爾蒙(Hormones), Viral
Antigens和各式抗體(antibody)

【ProSpec介紹】

ProSpec公司具有專業生產Recombinant Protein近二十年的經驗，其生產技術具有高產能、及高產率，因而生產成本低，ProSpec重組蛋白在市場上具有相當之競爭性。

Introduction	High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.
Description	HMG1 Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 223 amino acids and having a molecular mass of 26 kDa. The HMGB-1 is purified by proprietary chromatographic techniques.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	The HMG1 (1 mg/ml) was lyophilized after extensive dialyses against 1x PBS pH-7.4.
Solubility	It is recommended to reconstitute the lyophilized HMGB1 in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Stability	Lyophilized HMGB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HMGB1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity	Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Amino acid sequence	MGKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKT MSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFF LFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKY EKDIAAYRAKGKPDAAKKGVVKAEKSKKKEEEEDEEDEEDEEEEEDEEDEDE EEDDDDELEHHHHHH.

【ProSpec重組蛋白應用】

廣泛應用於研發、診斷試劑之開發、組織培養或無血清培養基之添加，癌症、細胞凋零、發育、內分泌、免疫、神經科學、蛋白質體、及幹細胞等相關領域的研究。

【ProSpec重組蛋白貯存】

產品均依冷凍乾燥方式供應，易於處理與儲存。

【供應廠商】太鼎生物科技有限公司

【連絡人】許虹宜0920-312382

【連絡信箱】taiding.biotech@msa.hinet.net

【臺北公司】02-86609496【台中公司】0935-229803 陳俊豪

【公司網址】 www.biopioneer.com.tw

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Protein A/G & Affinity Conjugates

產品描述

BioVision提供了獨特的重組蛋白A，G，L蛋白。重組蛋白A/G./L含有IgG結合區域，可以純化含有N-末端6X His-tag的蛋白質。已排除其它干擾區域之結合，例如細胞壁結合區域、白蛋白結合區域和其他非特異性結合區域。可確保達到最大特定IgG的含量。

---Protein A/G結合效能------

Protein A/G，結合ProteinA與Protein G蛋白。可純化大部份哺乳動物之IgG單株及多株抗體。

產品應用

1.
可應用於6XHis tag( N-末端)的親和純化

2.
蛋白質A / G/之檢測

檢測可使用抗His-tag抗體（biovision貨號＃3998-100）。

3.
純化多株抗體，或單株抗體IgG

產品特點

高品質的重組蛋白A，G和L由大腸桿菌中產生，並使用多重色層分析純化（未親合純化人的IgG）。及通過SDS-PAGE及HPLC純化，其純度> 98％。內毒素含量<0.05 EU/毫克。

蛋白質A，G和L可以結合,大多數哺乳動物, 之免疫球蛋白的Fc結合區域。因此，蛋白質A，G和L在純化多株抗體，或單株抗體IgG上，可純化抗體之含量。重組蛋白A / G和重組蛋白A / G/ L的組合都還可以，在抗體純化上都可以回收高量之抗體含量。

Protein A/G Affinity Conjugates

Catalog #	Product Name+	Size
6502-1	Protein A/G	1 mg
6502-100	Protein A/G	100 mg
6502-1000	Protein A/G	1 g
6502-25	Protein A/G	25 mg
6502-5	Protein A/G	5 mg

Protein A/G-FITC

Catalog #	Product Name+	Size
6504-01	Protein A/G-FITC	100 µg
6504-1	Protein A/G-FITC	1 mg

Protein A/G-Biotin

Catalog #	Product Name+	Size
6506-01	Protein A/G-Biotin	100 µg
6506-1	Protein A/G-Biotin	1 mg

Protein A/G-Sepharose

Catalog #	Product Name+	Size
6503-1	Protein A/G Sepharose	1 ml
6503-100	Protein A/G Sepharose	100 ml
6503-25	Protein A/G Sepharose	25 ml
6503-5	Protein A/G Sepharose	5 ml
6528-1	Protein A/G Sepharose Column	1 ml
6528-5	Protein A/G Sepharose Column	5 ml

Protein A/G-磁硃

Catalog #	Product Name+	Size
6527-1	Protein A/G Magnetic Beads	1 ml

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Protein A Affinity Conjugates

產品描述

BioVision提供了獨特的重組蛋白A，G，L蛋白。重組蛋白A/G./L含有IgG結合區域，可以純化含有N-末端6X His-tag的蛋白質。已排除其它干擾區域之結合，例如細胞壁結合區域、白蛋白結合區域和其他非特異性結合區域。可確保達到最大特定IgG的含量。

---Protein A結合效能------

Protein A為最佳純化單株抗體及多株抗體IgG。其適用於人類及Mouse IgG subclasses之純化 (例如human IgG1, IgG2, IgG4;
mouse IgG1, IgG2a, IgG2b, IgG3). 其也可以結合cow , guinea pig, hamster, horse, pig, 及 rabbit IgG含量. 在chicken, goat, rat 及 sheep.結合效能較低。

產品應用

1.
可應用於6XHis tag( N-末端)的親和純化

2.
蛋白質A / G/ L之檢測

檢測可使用抗His-tag抗體（biovision貨號＃3998-100）。

3.
純化多株抗體，或單株抗體IgG

產品特點

高品質的重組蛋白A，G和L由大腸桿菌中產生，並使用多重色層分析純化（未親合純化人的IgG）。及通過SDS-PAGE及HPLC純化，其純度> 98％。內毒素含量<0.05 EU/毫克。

蛋白質A，G和L可以結合,大多數哺乳動物, 之免疫球蛋白的Fc結合區域。因此，蛋白質A，G和L在純化多株抗體，或單株抗體IgG上，可純化抗體之含量。重組蛋白A / G和重組蛋白A / G/ L的組合都還可以，在抗體純化上都可以回收高量之抗體含量。

Protein A Affinity Conjugates

Catalog #	Product Name+	Size
6500-10	Protein A (Liquid form)	10 mg
6500-100	Protein A (Liquid form)	100 mg
6500-1000	Protein A (Liquid form)	1 g
6500-25	Protein A (Liquid form)	25 mg
6500B-10	Protein A (Lyophilized)	10 mg
6500B-100	Protein A (Lyophilized)	100 mg
6500B-1000	Protein A (Lyophilized)	1 g
6500B-25	Protein A (Lyophilized)	25 mg

Protein A 抗體

Catalog #	Product Name+	Size
5500-100	Protein A Antibody	100 µg

Protein A Sepharose

Catalog #	Product Name+	Size
6501-1	Protein A Sepharose	1 ml
6501-100	Protein A Sepharose	100 ml
6501-25	Protein A Sepharose	25 ml
6501-5	Protein A Sepharose	5 ml
6508-1	Protein A Sepharose Column	1 ml
6508-5	Protein A Sepharose Column	5 ml

Protein A 磁硃

在磁珠上連接有 Protein A 層析膠體的純化產品，可簡化免疫沈澱或抗體純化流程，加速實驗進行。

Catalog #	Product Name+	Size
6507-1	Protein A Magnetic Beads	1 ml

Protein A Agarose

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Anti-TGFβ1 抗體

應用於人類、mouse及rat物種；應用IHC測試，確定可用於IHC實驗。建議使用量1:50~1:200倍稀釋。

TGF beta1 Antibody

Catalog #	Product Name+	Size
6520-1	Hi-Bind™ Protein A-Agarose	1 ml
6520-100	Hi-Bind™ Protein A-Agarose	100 ml
6520-25	Hi-Bind™ Protein A-Agarose	25 ml
6520-5	Hi-Bind™ Protein A-Agarose	5 ml
6526-1	Protein A-Agarose

Antibody Type	primary antibody
Accession #	NM_000660
Description	TGF beta1 is a TGFB is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA (MIM 190170) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for this peptide. TGFB1, TGFB2 (MIM 190220), and TGFB3 (MIM 190230) all function through the same receptor signaling systems.[
Immunogen	partial recombinate protein
Species Reactivity	human,mus,rattus
Specificity	TGF beta1 antibody detects endogenous levels of TGF1 beta1 protein. Not tested with other proteins
Tested Applications	Peptide ELISAWB:1:500 IHC
Application Notes	Recommended dilutions Peptide ELISA: 1:80,000 WB: 1/200 - 1/1000 IHc:1/50-1/200 Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user
Raised In	Rabbit
Clonality	Polyclonal
Purity	Protein A purified
Storage Buffer	Rabbit IgG containing 0.02% sodium azide
Formulation	Liquid
Concentration	100ug/100ul
Storage	Store at -20°C /1 year
References	Korff,T., Aufgebauer,K. and Hecker,M Cyclic stretch controls the expression of CD 40 in endothelial cells by changing their transforming growth factor-beta1 response Circulation 2007:116 (20), 2288-2297
Caution	TGF beta1 is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

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Host:	Chicken
Volume:	0.2 ml
Quantity:	0.1 mg
Type:	HRP conjugated
Full Name:	HRP-conjugated Affinity Purified Chicken anti-Digoxigenin
Physical State:	liquid
Concentration:	0.5 mg/ml
Diluent:	Hepes-Saline with 0.1% BSA, 50% Glycerol
Preservative:	0.005% Thimerosal
Procedure:	Chickens were immunized with Digoxigenin coupled to KLH. After multiple immunizations in Freund's adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the Digoxigenin immobilized on a solid support and conjugated to horseradish peroxidase (HRP).
Specifications:	This antibody is suitable for ELISA and Western blots. Recommended dilution for Western blots is 1:500. For ELISA, recommended dilution is 1:5,000.
Storage:	4°C or -20°C for long-term storage. Don't freeze-thaw often.
Expiration Date:	0000-00-00
Note:	For research use only.

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IgY Eggs Press Purification Kit

IgY抗體純化---產品描述

產品規格

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Endotoxin Assay Kits

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IgY Eggs Press
Purification Kit